This is a Validated Antibody Database (VAD) review about Saccharomy.. VMA2, based on 40 published articles (read how Labome selects the articles), using VMA2 antibody in all methods. It is aimed to help Labome visitors find the most suited VMA2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
VMA2 synonym: VAT2; H(+)-transporting V1 sector ATPase subunit B

Invitrogen
mouse monoclonal (13D11B2)
  • western blot; baker's yeast; 1:4000
In order to study Leishmania LHR1 and heme uptake in Saccharomyces cerevisiae, Invitrogen VMA2 antibody (Life Technologies, A-6427) was used in western blot on baker's yeast samples at 1:4000. PLoS Negl Trop Dis (2015) ncbi
mouse monoclonal (13D11B2)
In order to elucidate the signaling pathways involved in maintaining cell wall integrity, Invitrogen VMA2 antibody (Invitrogen, 13D11B2) was used . FEBS Lett (2013) ncbi
mouse monoclonal (13D11B2)
In order to model the N-terminal domain of Vph1p and test this model, Invitrogen VMA2 antibody (Invitrogen, clone 13D11) was used . J Biol Chem (2013) ncbi
mouse monoclonal (13D11B2)
In order to report a pharmacological strategy that reduces signs of aging in and increases the lifespan of Saccharomyces cerevisiae, Invitrogen VMA2 antibody (Invitrogen, A6427) was used . Aging Cell (2013) ncbi
mouse monoclonal (13D11B2)
In order to construct and experimentally test a predictive model of the membrane insertion of the yeast a subunit, Vph1p, Invitrogen VMA2 antibody (Invitrogen, 13D11) was used . J Cell Biochem (2013) ncbi
mouse monoclonal (13D11B2)
In order to test if cells lacking BTN1 have altered membrane phospholipid content, Invitrogen VMA2 antibody (Molecular Probes, 13D11) was used . Dis Model Mech (2012) ncbi
mouse monoclonal (13D11B2)
In order to describe the hemichannel of H(+)-ATPases, Invitrogen VMA2 antibody (Molecular Probes, clone 13D11) was used . J Biol Chem (2011) ncbi
mouse monoclonal (13D11B2)
  • western blot; baker's yeast; fig 3
In order to identify the most recent common ancestor of Vph1p and Stv1p and assess its function in yeast, Invitrogen VMA2 antibody (Molecular Probes, 13D11B2) was used in western blot on baker's yeast samples (fig 3). Mol Biol Cell (2011) ncbi
mouse monoclonal (13D11B2)
  • western blot; fission yeast; fig 5
In order to identify the SEA complex is a member of a family of membrane coating and vesicle tethering assemblies needed for intracellular trafficking, amino acid biogenesis, and response to nitrogen starvation, Invitrogen VMA2 antibody (Invitrogen, A-6427) was used in western blot on fission yeast samples (fig 5). Mol Cell Proteomics (2011) ncbi
mouse monoclonal (13D11B2)
  • western blot; baker's yeast; 1:5000; fig 3
In order to report how extracellular pH alterations affect Btn1p, Invitrogen VMA2 antibody (Molecular Probes, 13D11) was used in western blot on baker's yeast samples at 1:5000 (fig 3). Dis Model Mech (2011) ncbi
mouse monoclonal (13D11B2)
  • western blot; baker's yeast; fig 7
In order to identify important functional residues of subunit a of V-ATPases, Invitrogen VMA2 antibody (Invitrogen, 13D11) was used in western blot on baker's yeast samples (fig 7). J Biol Chem (2009) ncbi
mouse monoclonal (13D11B2)
  • western blot; baker's yeast; 1:1000
In order to describe the properties of V-ATPase complexes, Invitrogen VMA2 antibody (Molecular Probes, 13D11) was used in western blot on baker's yeast samples at 1:1000. J Biol Chem (2008) ncbi
mouse monoclonal (13D11B2)
  • immunocytochemistry; baker's yeast
In order to characterize the properties of E1/yeast hybrid V-ATPase, Invitrogen VMA2 antibody (Molecular Probes, 13D11) was used in immunocytochemistry on baker's yeast samples . Biochim Biophys Acta (2008) ncbi
mouse monoclonal (13D11B2)
  • immunoprecipitation; baker's yeast; fig 1
In order to investigate the actin-binding activity of the B subunit of yeast V-ATPase, Invitrogen VMA2 antibody (Molecular Probes, 13D11) was used in immunoprecipitation on baker's yeast samples (fig 1). J Exp Biol (2008) ncbi
mouse monoclonal (13D11B2)
  • western blot; cow; fig 1b
In order to assess the expression, localization, and functionality of vacuolar-type H(+)-ATPase and Na(+)/K(+)-ATPase in the bovine rumen epithelium, Invitrogen VMA2 antibody (Molecular Probes, 13D11-B2) was used in western blot on cow samples (fig 1b). J Comp Physiol B (2008) ncbi
mouse monoclonal (13D11B2)
  • western blot; baker's yeast; fig 1b
In order to report that cell wall integrity microtubule-associated protein kinase kinases are targets for their downstream MAPK, Invitrogen VMA2 antibody (Molecular Probes, 13D11) was used in western blot on baker's yeast samples (fig 1b). J Biol Chem (2007) ncbi
mouse monoclonal (13D11B2)
  • western blot; baker's yeast
In order to study the result in osteopetrosis and distal renal tubular acidosis on yeast V-ATPase activity and expression due to effects of human a3 and a4 mutations, Invitrogen VMA2 antibody (Molecular Probes, 13D-11) was used in western blot on baker's yeast samples . J Biol Chem (2006) ncbi
mouse monoclonal (13D11B2)
  • western blot; sheep; fig 7
In order to identify and characterize a vacuolar-type H+-ATPase in sheep ruminal epithelial cells, Invitrogen VMA2 antibody (Molecular Probes, 13D11-B2) was used in western blot on sheep samples (fig 7). Am J Physiol Gastrointest Liver Physiol (2006) ncbi
mouse monoclonal (13D11B2)
  • western blot; baker's yeast
In order to propose that the dependence of the dissociation of the V-ATPase on catalytic activity is due to neutralization of the yeast vacuole, Invitrogen VMA2 antibody (Molecular Probes, 13D11-B2) was used in western blot on baker's yeast samples . J Biol Chem (2004) ncbi
mouse monoclonal (13D11B2)
In order to investigate how ATP is supplied for V-ATPase-mediated hydrolysis and for coupling of proton transport, Invitrogen VMA2 antibody (Molecular Probes, 13D11) was used . J Biol Chem (2004) ncbi
mouse monoclonal (13D11B2)
  • immunocytochemistry; baker's yeast; fig 5D
In order to study and characterize organelle-specific myosin V receptor, Invitrogen VMA2 antibody (Molecular Probes, noca) was used in immunocytochemistry on baker's yeast samples (fig 5D). J Cell Biol (2003) ncbi
mouse monoclonal (13D11B2)
  • immunoprecipitation; baker's yeast; fig 6
In order to identify proteins that bind E subunit of V-ATPase, Invitrogen VMA2 antibody (Molecular Probes, A6427) was used in immunoprecipitation on baker's yeast samples (fig 6). J Biol Chem (2002) ncbi
mouse monoclonal (13D11B2)
In order to develop a new probe to study the yeast secretory system, Invitrogen VMA2 antibody (Molecular Probes, 13D11-B2) was used . J Biol Chem (2002) ncbi
mouse monoclonal (13D11B2)
In order to determine the results of Mns1p and Rer1p interactions, Invitrogen VMA2 antibody (Molecular Probes, clone 13D11-B2) was used . J Cell Sci (2001) ncbi
mouse monoclonal (13D11B2)
In order to screen for genes required for the slow delivery protein pathway using yeast, Invitrogen VMA2 antibody (Molecular Probes, 13D11-B2) was used . Mol Biol Cell (2001) ncbi
mouse monoclonal (13D11B2)
In order to identify the residues required for proton transport by the V-ATPases, Invitrogen VMA2 antibody (Molecular Probes, 13D11-B2) was used . Proc Natl Acad Sci U S A (2001) ncbi
mouse monoclonal (13D11B2)
In order to examine the role of "a" subunit of the vacuolar proton-translocating ATPase in cellular localization of the complex, Invitrogen VMA2 antibody (Molecular Probes, 13D11-B2) was used . J Biol Chem (2001) ncbi
mouse monoclonal (13D11B2)
In order to study the role of Vps35p in transporting cargo proteins, Invitrogen VMA2 antibody (Molecular Probes, 13D11-B2) was used . J Cell Biol (2000) ncbi
mouse monoclonal (13D11B2)
  • immunocytochemistry; baker's yeast; fig 3
In order to examine the regulation and reversibility of V-ATPase, Invitrogen VMA2 antibody (Molecular Probes, A6427) was used in immunocytochemistry on baker's yeast samples (fig 3). J Biol Chem (2000) ncbi
mouse monoclonal (13D11B2)
In order to identify residues involved in the formation of the noncatalytic nucleotide binding sites of the vacuolar proton-translocating adenosine triphosphatase, Invitrogen VMA2 antibody (Molecular Probes, 13D11) was used . J Biol Chem (2000) ncbi
mouse monoclonal (13D11B2)
In order to elucidate how the vacuole contributes to iron metabolism, Invitrogen VMA2 antibody (Molecular Probes, 13D11) was used . J Biol Chem (1999) ncbi
mouse monoclonal (13D11B2)
In order to identify GRD20 as a gene that is important for protein sorting in the TGN/endosomal system of Saccharomyces cerevisiae, Invitrogen VMA2 antibody (Molecular Probes, 13D11-B2) was used . Mol Biol Cell (1999) ncbi
mouse monoclonal (13D11B2)
  • western blot; baker's yeast; fig 2
In order to study nucleotide- and cation- induced changes in the catalytic core of the V-ATPase, Invitrogen VMA2 antibody (Molecular Probes, 13D11-B2) was used in western blot on baker's yeast samples (fig 2). J Biol Chem (1999) ncbi
mouse monoclonal (13D11B2)
  • western blot; baker's yeast; fig 3
In order to elucidate proton translocation by the vacuolar H(+)-ATPase, Invitrogen VMA2 antibody (Molecular Probes, noca) was used in western blot on baker's yeast samples (fig 3). J Biol Chem (1999) ncbi
mouse monoclonal (13D11B2)
In order to report that loss of Vps35p function results in mislocalization of A-ALP to the vacuole, Invitrogen VMA2 antibody (Molecular Probes, 13D11-B2) was used . Mol Biol Cell (1999) ncbi
mouse monoclonal (13D11B2)
  • western blot; baker's yeast; 1:1000; fig 1
In order to characterize Vma12p, Invitrogen VMA2 antibody (Molecular Probes, 13D11-B2) was used in western blot on baker's yeast samples at 1:1000 (fig 1). J Biol Chem (1997) ncbi
mouse monoclonal (13D11B2)
  • immunocytochemistry; baker's yeast; fig 5
In order to suggest that Vps28p facilitates the formation of transport intermediates required for efficient transport out of the prevacuolar endosome, Invitrogen VMA2 antibody (Molecular Probes, noca) was used in immunocytochemistry on baker's yeast samples (fig 5). Mol Biol Cell (1996) ncbi
mouse monoclonal (13D11B2)
  • immunocytochemistry; baker's yeast; 10 ng/ml; fig 3D
In order to determine the structural features and intercompartmental transport of carboxypeptidase Y, Invitrogen VMA2 antibody (Molecular Probes, noca) was used in immunocytochemistry on baker's yeast samples at 10 ng/ml (fig 3D). Mol Biol Cell (1995) ncbi
mouse monoclonal (13D11B2)
  • immunocytochemistry; baker's yeast; fig 4C
In order to suggest that Vps27p controls membrane traffic through the prevacuolar/endosomal compartment, Invitrogen VMA2 antibody (Molecular Probes, 13D11-B2) was used in immunocytochemistry on baker's yeast samples (fig 4C). J Cell Biol (1995) ncbi
mouse monoclonal (13D11B2)
  • immunocytochemistry; baker's yeast; fig 1
In order to analyze sorting defects in vacuolar protein sorting mutants in Saccharomyces cerevisiae, Invitrogen VMA2 antibody (noco, noca) was used in immunocytochemistry on baker's yeast samples (fig 1). Mol Biol Cell (1992) ncbi
Articles Reviewed
  1. Renberg R, Yuan X, Samuel T, Miguel D, Hamza I, Andrews N, et al. The Heme Transport Capacity of LHR1 Determines the Extent of Virulence in Leishmania amazonensis. PLoS Negl Trop Dis. 2015;9:e0003804 pubmed publisher
  2. Rodríguez Peña J, Diez Muñiz S, Bermejo C, Nombela C, Arroyo J. Activation of the yeast cell wall integrity MAPK pathway by zymolyase depends on protease and glucanase activities and requires the mucin-like protein Hkr1 but not Msb2. FEBS Lett. 2013;587:3675-80 pubmed publisher
  3. Liberman R, Cotter K, Baleja J, Forgac M. Structural analysis of the N-terminal domain of subunit a of the yeast vacuolar ATPase (V-ATPase) using accessibility of single cysteine substitutions to chemical modification. J Biol Chem. 2013;288:22798-808 pubmed publisher
  4. Huang X, Liu J, Withers B, Samide A, Leggas M, Dickson R. Reducing signs of aging and increasing lifespan by drug synergy. Aging Cell. 2013;12:652-60 pubmed publisher
  5. Kartner N, Yao Y, Bhargava A, Manolson M. Topology, glycosylation and conformational changes in the membrane domain of the vacuolar H+-ATPase a subunit. J Cell Biochem. 2013;114:1474-87 pubmed publisher
  6. Padilla Lopez S, Langager D, Chan C, Pearce D. BTN1, the Saccharomyces cerevisiae homolog to the human Batten disease gene, is involved in phospholipid distribution. Dis Model Mech. 2012;5:191-9 pubmed publisher
  7. Toei M, Toei S, Forgac M. Definition of membrane topology and identification of residues important for transport in subunit a of the vacuolar ATPase. J Biol Chem. 2011;286:35176-86 pubmed publisher
  8. Finnigan G, Hanson Smith V, Houser B, Park H, Stevens T. The reconstructed ancestral subunit a functions as both V-ATPase isoforms Vph1p and Stv1p in Saccharomyces cerevisiae. Mol Biol Cell. 2011;22:3176-91 pubmed publisher
  9. Dokudovskaya S, Waharte F, Schlessinger A, Pieper U, Devos D, Cristea I, et al. A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae. Mol Cell Proteomics. 2011;10:M110.006478 pubmed publisher
  10. Wolfe D, Padilla Lopez S, Vitiello S, Pearce D. pH-dependent localization of Btn1p in the yeast model for Batten disease. Dis Model Mech. 2011;4:120-5 pubmed publisher
  11. Ediger B, Melman S, Pappas D, Finch M, Applen J, Parra K. The tether connecting cytosolic (N terminus) and membrane (C terminus) domains of yeast V-ATPase subunit a (Vph1) is required for assembly of V0 subunit d. J Biol Chem. 2009;284:19522-32 pubmed publisher
  12. Flannery A, Stevens T. Functional characterization of the N-terminal domain of subunit H (Vma13p) of the yeast vacuolar ATPase. J Biol Chem. 2008;283:29099-108 pubmed publisher
  13. Hayashi K, Sun Wada G, Wada Y, Nakanishi Matsui M, Futai M. Defective assembly of a hybrid vacuolar H(+)-ATPase containing the mouse testis-specific E1 isoform and yeast subunits. Biochim Biophys Acta. 2008;1777:1370-7 pubmed publisher
  14. Zuo J, Vergara S, Kohno S, Holliday L. Biochemical and functional characterization of the actin-binding activity of the B subunit of yeast vacuolar H+-ATPase. J Exp Biol. 2008;211:1102-8 pubmed publisher
  15. Albrecht E, Kolisek M, Viergutz T, Zitnan R, Schweigel M. Molecular identification, immunolocalization, and functional activity of a vacuolar-type H(+)-ATPase in bovine rumen epithelium. J Comp Physiol B. 2008;178:285-95 pubmed
  16. Jimenez Sanchez M, Cid V, Molina M. Retrophosphorylation of Mkk1 and Mkk2 MAPKKs by the Slt2 MAPK in the yeast cell integrity pathway. J Biol Chem. 2007;282:31174-85 pubmed
  17. Ochotny N, Van Vliet A, Chan N, Yao Y, Morel M, Kartner N, et al. Effects of human a3 and a4 mutations that result in osteopetrosis and distal renal tubular acidosis on yeast V-ATPase expression and activity. J Biol Chem. 2006;281:26102-11 pubmed
  18. Etschmann B, Heipertz K, von der Schulenburg A, Schweigel M. A vH+-ATPase is present in cultured sheep ruminal epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2006;291:G1171-9 pubmed
  19. Shao E, Forgac M. Involvement of the nonhomologous region of subunit A of the yeast V-ATPase in coupling and in vivo dissociation. J Biol Chem. 2004;279:48663-70 pubmed
  20. Lu M, Sautin Y, Holliday L, Gluck S. The glycolytic enzyme aldolase mediates assembly, expression, and activity of vacuolar H+-ATPase. J Biol Chem. 2004;279:8732-9 pubmed
  21. Ishikawa K, Catlett N, Novak J, Tang F, Nau J, Weisman L. Identification of an organelle-specific myosin V receptor. J Cell Biol. 2003;160:887-97 pubmed
  22. Lu M, Vergara S, Zhang L, Holliday L, Aris J, Gluck S. The amino-terminal domain of the E subunit of vacuolar H(+)-ATPase (V-ATPase) interacts with the H subunit and is required for V-ATPase function. J Biol Chem. 2002;277:38409-15 pubmed
  23. Ferreira T, Mason A, Pypaert M, Allen K, Slayman C. Quality control in the yeast secretory pathway: a misfolded PMA1 H+-ATPase reveals two checkpoints. J Biol Chem. 2002;277:21027-40 pubmed
  24. Massaad M, Herscovics A. Interaction of the endoplasmic reticulum alpha 1,2-mannosidase Mns1p with Rer1p using the split-ubiquitin system. J Cell Sci. 2001;114:4629-35 pubmed
  25. Ha S, Bunch J, Hama H, DeWald D, Nothwehr S. A novel mechanism for localizing membrane proteins to yeast trans-Golgi network requires function of synaptojanin-like protein. Mol Biol Cell. 2001;12:3175-90 pubmed
  26. Kawasaki Nishi S, Nishi T, Forgac M. Arg-735 of the 100-kDa subunit a of the yeast V-ATPase is essential for proton translocation. Proc Natl Acad Sci U S A. 2001;98:12397-402 pubmed
  27. Kawasaki Nishi S, Bowers K, Nishi T, Forgac M, Stevens T. The amino-terminal domain of the vacuolar proton-translocating ATPase a subunit controls targeting and in vivo dissociation, and the carboxyl-terminal domain affects coupling of proton transport and ATP hydrolysis. J Biol Chem. 2001;276:47411-20 pubmed
  28. Nothwehr S, Ha S, Bruinsma P. Sorting of yeast membrane proteins into an endosome-to-Golgi pathway involves direct interaction of their cytosolic domains with Vps35p. J Cell Biol. 2000;151:297-310 pubmed
  29. Hirata T, Nakamura N, Omote H, Wada Y, Futai M. Regulation and reversibility of vacuolar H(+)-ATPase. J Biol Chem. 2000;275:386-9 pubmed
  30. Vasilyeva E, Liu Q, MacLeod K, Baleja J, Forgac M. Cysteine scanning mutagenesis of the noncatalytic nucleotide binding site of the yeast V-ATPase. J Biol Chem. 2000;275:255-60 pubmed
  31. Urbanowski J, Piper R. The iron transporter Fth1p forms a complex with the Fet5 iron oxidase and resides on the vacuolar membrane. J Biol Chem. 1999;274:38061-70 pubmed
  32. Spelbrink R, Nothwehr S. The yeast GRD20 gene is required for protein sorting in the trans-Golgi network/endosomal system and for polarization of the actin cytoskeleton. Mol Biol Cell. 1999;10:4263-81 pubmed
  33. Landolt Marticorena C, Kahr W, Zawarinski P, Correa J, Manolson M. Substrate- and inhibitor-induced conformational changes in the yeast V-ATPase provide evidence for communication between the catalytic and proton-translocating sectors. J Biol Chem. 1999;274:26057-64 pubmed
  34. Harrison M, Murray J, Powell B, Kim Y, Finbow M, Findlay J. Helical interactions and membrane disposition of the 16-kDa proteolipid subunit of the vacuolar H(+)-ATPase analyzed by cysteine replacement mutagenesis. J Biol Chem. 1999;274:25461-70 pubmed
  35. Nothwehr S, Bruinsma P, Strawn L. Distinct domains within Vps35p mediate the retrieval of two different cargo proteins from the yeast prevacuolar/endosomal compartment. Mol Biol Cell. 1999;10:875-90 pubmed
  36. Jackson D, Stevens T. VMA12 encodes a yeast endoplasmic reticulum protein required for vacuolar H+-ATPase assembly. J Biol Chem. 1997;272:25928-34 pubmed
  37. Rieder S, Banta L, Köhrer K, McCaffery J, Emr S. Multilamellar endosome-like compartment accumulates in the yeast vps28 vacuolar protein sorting mutant. Mol Biol Cell. 1996;7:985-99 pubmed
  38. Cereghino J, Marcusson E, Emr S. The cytoplasmic tail domain of the vacuolar protein sorting receptor Vps10p and a subset of VPS gene products regulate receptor stability, function, and localization. Mol Biol Cell. 1995;6:1089-102 pubmed
  39. Piper R, Cooper A, Yang H, Stevens T. VPS27 controls vacuolar and endocytic traffic through a prevacuolar compartment in Saccharomyces cerevisiae. J Cell Biol. 1995;131:603-17 pubmed
  40. Raymond C, Howald Stevenson I, Vater C, Stevens T. Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell. 1992;3:1389-402 pubmed