This is a Validated Antibody Database (VAD) review about Saccharomy.. PEP12, based on 5 published articles (read how Labome selects the articles), using PEP12 antibody in all methods. It is aimed to help Labome visitors find the most suited PEP12 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PEP12 synonym: VPL6; VPS6; VPT13; SNAP receptor PEP12

Invitrogen
rabbit monoclonal (7HCLC)
  • western blot; baker's yeast; 1:1000; fig s2
In order to study initiation of autophagy by tethering Atg9-vesicles by the Atg1-kinase complex, Invitrogen PEP12 antibody (Invitrogen, 710037) was used in western blot on baker's yeast samples at 1:1000 (fig s2). Nat Commun (2016) ncbi
mouse monoclonal (2C3G4)
  • western blot; budding yeasts; fig 3
In order to describe a method for monitoring intact autophagosomes ex vivo, Invitrogen PEP12 antibody (Invitrogen, A21273) was used in western blot on budding yeasts samples (fig 3). PLoS ONE (2014) ncbi
mouse monoclonal (2C3G4)
  • western blot; baker's yeast; 1:2000
In order to investigate the effects of sertraline/Zoloft on the cell physiology of Saccharomyces cerevisiae, Invitrogen PEP12 antibody (Invitrogen, A-21273) was used in western blot on baker's yeast samples at 1:2000. PLoS ONE (2012) ncbi
mouse monoclonal (2C3G4)
  • immunocytochemistry; baker's yeast; fig 2
In order to study the accumulation of phosphatidylinositol 4,5-bisphosphate on vacuolar fusion defects and vacuole membranes by inactivation of the phosphoinositide phosphatases Sac1p and Inp54p, Invitrogen PEP12 antibody (Molecular Probes, noca) was used in immunocytochemistry on baker's yeast samples (fig 2). J Biol Chem (2007) ncbi
mouse monoclonal (2C3G4)
  • immunocytochemistry; baker's yeast; fig 3
  • western blot; baker's yeast; fig 2
In order to determine the role of Pep12p in vacuolar protein transport, Invitrogen PEP12 antibody (Molecular Probes, 2C3G4) was used in immunocytochemistry on baker's yeast samples (fig 3) and in western blot on baker's yeast samples (fig 2). Traffic (2000) ncbi
Articles Reviewed
  1. Rao Y, Perna M, Hofmann B, Beier V, Wollert T. The Atg1-kinase complex tethers Atg9-vesicles to initiate autophagy. Nat Commun. 2016;7:10338 pubmed publisher
  2. Suzuki K, Nakamura S, Morimoto M, Fujii K, Noda N, Inagaki F, et al. Proteomic profiling of autophagosome cargo in Saccharomyces cerevisiae. PLoS ONE. 2014;9:e91651 pubmed publisher
  3. Chen J, Korostyshevsky D, Lee S, Perlstein E. Accumulation of an antidepressant in vesiculogenic membranes of yeast cells triggers autophagy. PLoS ONE. 2012;7:e34024 pubmed publisher
  4. Wiradjaja F, Ooms L, Tahirovic S, Kuhne E, Devenish R, Munn A, et al. Inactivation of the phosphoinositide phosphatases Sac1p and Inp54p leads to accumulation of phosphatidylinositol 4,5-bisphosphate on vacuole membranes and vacuolar fusion defects. J Biol Chem. 2007;282:16295-307 pubmed
  5. Gerrard S, Mecklem A, Stevens T. The yeast endosomal t-SNARE, Pep12p, functions in the absence of its transmembrane domain. Traffic. 2000;1:45-55 pubmed