This is a Validated Antibody Database (VAD) review about Rhesus mon.. SIRPA, based on 10 published articles (read how Labome selects the articles), using SIRPA antibody in all methods. It is aimed to help Labome visitors find the most suited SIRPA antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SIRPA synonym: tyrosine-protein phosphatase non-receptor type substrate 1

BioLegend
mouse monoclonal (SE5A5)
  • flow cytometry; rat; loading ...; fig 1a
BioLegend SIRPA antibody (Biolegend, 323810) was used in flow cytometry on rat samples (fig 1a). Stem Cell Reports (2018) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; loading ...; fig 1e
BioLegend SIRPA antibody (BioLegend, SE5A5) was used in flow cytometry on human samples (fig 1e). J Exp Med (2018) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; loading ...; fig s2a
BioLegend SIRPA antibody (BioLegend, SE5A5) was used in flow cytometry on human samples (fig s2a). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; fig 2f
In order to map the lineage of human dendritic cells, BioLegend SIRPA antibody (BioLegend, SE5a5) was used in flow cytometry on human samples (fig 2f). Science (2017) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; loading ...; fig s1b
In order to determine the localization of FcgammaRI, FcgammaRII, and SIRPalpha in macrophages, BioLegend SIRPA antibody (BioLegend, SE5A5) was used in flow cytometry on human samples (fig s1b). J Cell Biol (2017) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; 1:1000; loading ...; fig s1c
In order to describe a protocol to generate sinoatrial node-like pacemaker cells, BioLegend SIRPA antibody (BioLegend, 323807) was used in flow cytometry on human samples at 1:1000 (fig s1c). Nat Biotechnol (2017) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; 1:500; fig 1c
In order to study the role of the RAS/MAPK pathway in hypertrophic cardiomyopathy, BioLegend SIRPA antibody (BioLegend, 323807) was used in flow cytometry on human samples at 1:500 (fig 1c). Stem Cell Reports (2016) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BioLegend SIRPA antibody (BioLegen d, 323806) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; 1:500; fig 2g
In order to optimize conditions to promote the proliferation of multipotent cardiovascular progenitor cells, BioLegend SIRPA antibody (Biolegend, 323801) was used in flow cytometry on human samples at 1:500 (fig 2g). Nat Biotechnol (2015) ncbi
mouse monoclonal (SE5A5)
  • flow cytometry; human; fig 3
In order to study human cord blood and bone marrow for restricted dendritic cell and monocyte progenitors, BioLegend SIRPA antibody (Biolegend, SE5A5) was used in flow cytometry on human samples (fig 3). J Exp Med (2015) ncbi
Articles Reviewed
  1. Yang X, Zhou J, He J, Liu J, Wang H, Liu Y, et al. An Immune System-Modified Rat Model for Human Stem Cell Transplantation Research. Stem Cell Reports. 2018;11:514-521 pubmed publisher
  2. Bujko A, Atlasy N, Landsverk O, Richter L, Yaqub S, Horneland R, et al. Transcriptional and functional profiling defines human small intestinal macrophage subsets. J Exp Med. 2018;215:441-458 pubmed publisher
  3. Ring N, Herndler Brandstetter D, Weiskopf K, Shan L, Volkmer J, George B, et al. Anti-SIRP? antibody immunotherapy enhances neutrophil and macrophage antitumor activity. Proc Natl Acad Sci U S A. 2017;114:E10578-E10585 pubmed publisher
  4. See P, Dutertre C, Chen J, Günther P, McGovern N, Irac S, et al. Mapping the human DC lineage through the integration of high-dimensional techniques. Science. 2017;356: pubmed publisher
  5. Lopes F, Bálint Å, Valvo S, Felce J, Hessel E, Dustin M, et al. Membrane nanoclusters of FcγRI segregate from inhibitory SIRPα upon activation of human macrophages. J Cell Biol. 2017;216:1123-1141 pubmed publisher
  6. Protze S, Liu J, Nussinovitch U, Ohana L, Backx P, Gepstein L, et al. Sinoatrial node cardiomyocytes derived from human pluripotent cells function as a biological pacemaker. Nat Biotechnol. 2017;35:56-68 pubmed publisher
  7. Josowitz R, Mulero Navarro S, Rodriguez N, Falce C, Cohen N, Ullian E, et al. Autonomous and Non-autonomous Defects Underlie Hypertrophic Cardiomyopathy in BRAF-Mutant hiPSC-Derived Cardiomyocytes. Stem Cell Reports. 2016;7:355-369 pubmed publisher
  8. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  9. Birket M, Ribeiro M, Verkerk A, Ward D, Leitoguinho A, Den Hartogh S, et al. Expansion and patterning of cardiovascular progenitors derived from human pluripotent stem cells. Nat Biotechnol. 2015;33:970-9 pubmed publisher
  10. Lee J, Breton G, Oliveira T, Zhou Y, Aljoufi A, PUHR S, et al. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J Exp Med. 2015;212:385-99 pubmed publisher