This is a Validated Antibody Database (VAD) review about Drosophila.. alpha-Spec, based on 8 published articles (read how Labome selects the articles), using alpha-Spec antibody in all methods. It is aimed to help Labome visitors find the most suited alpha-Spec antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
alpha-Spec synonym: 3A9; CG1977; DmelCG1977; FBtr0072789; FCP-B; SPTCA; Sp; Spec; Spectrin; alfa-Spec; alpha; alpha spectrin; alpha-SPEC; alpha-Sp; alpha-Spc; alpha-Spe; alpha-Spect; alpha-Spectrin; alpha-spec; alpha-spectrin; alphaSp; alphaSpec; alphaSpectrin; anon-EST:fe2E2; dre3; klo; l(3)04276; l(3)62Bd; l(3)alpha-Spec; l(3)dre3; spectrin

Developmental Studies Hybridoma Bank
mouse monoclonal (3A9 (323 or M10-2))
  • immunohistochemistry; Drosophila melanogaster; 1:100; loading ...; fig 2s1i
Developmental Studies Hybridoma Bank alpha-Spec antibody (DSHB, 3A9) was used in immunohistochemistry on Drosophila melanogaster samples at 1:100 (fig 2s1i). elife (2019) ncbi
mouse monoclonal (3A9 (323 or M10-2))
  • immunohistochemistry - paraffin section; Drosophila melanogaster; 1:200; loading ...; fig 7f
Developmental Studies Hybridoma Bank alpha-Spec antibody (Developmental Studies Hybridoma Bank, 3A9) was used in immunohistochemistry - paraffin section on Drosophila melanogaster samples at 1:200 (fig 7f). Neuron (2018) ncbi
mouse monoclonal (3A9 (323 or M10-2))
  • immunohistochemistry; Drosophila melanogaster; 1:25; loading ...; tbl 3
Developmental Studies Hybridoma Bank alpha-Spec antibody (DSHB, 3A9) was used in immunohistochemistry on Drosophila melanogaster samples at 1:25 (tbl 3). elife (2015) ncbi
mouse monoclonal (3A9 (323 or M10-2))
  • immunohistochemistry; Drosophila melanogaster; 1:25
In order to describe a new method to generate designer genes: Gene targeting during Oogenesis with Lethality Inhibitor and CRISPR/Cas (Golic+), Developmental Studies Hybridoma Bank alpha-Spec antibody (DSHB, 3A9) was used in immunohistochemistry on Drosophila melanogaster samples at 1:25. Genetics (2015) ncbi
mouse monoclonal (3A9 (323 or M10-2))
  • immunohistochemistry; Drosophila melanogaster; 1:5
In order to study Nasrat and Polehole during development, Developmental Studies Hybridoma Bank alpha-Spec antibody (Developmental Studies Hybridoma Bank, 3A9) was used in immunohistochemistry on Drosophila melanogaster samples at 1:5. Fly (Austin) (2014) ncbi
mouse monoclonal (3A9 (323 or M10-2))
  • immunocytochemistry; Drosophila melanogaster; 1:50
In order to determine the contribution of Tut, Bam, and Bgcn in the proliferation and differentiation of the Drosophila germline stem cell lineage, Developmental Studies Hybridoma Bank alpha-Spec antibody (DSHB, 3A9) was used in immunocytochemistry on Drosophila melanogaster samples at 1:50. PLoS Genet (2014) ncbi
mouse monoclonal (3A9 (323 or M10-2))
  • immunohistochemistry; Drosophila melanogaster; 1:20; fig 1
In order to study a stress assembly that supports cell viability by preserving ERES components during amino-acid starvation, Developmental Studies Hybridoma Bank alpha-Spec antibody (DSHB, 3A9) was used in immunohistochemistry on Drosophila melanogaster samples at 1:20 (fig 1). elife (2014) ncbi
mouse monoclonal (3A9 (323 or M10-2))
  • immunohistochemistry - free floating section; Drosophila melanogaster; 1:20
  • western blot; Drosophila melanogaster; 1:1000-10,000
Developmental Studies Hybridoma Bank alpha-Spec antibody (The Developmental Studies Hybridoma Bank, 3A9) was used in immunohistochemistry - free floating section on Drosophila melanogaster samples at 1:20 and in western blot on Drosophila melanogaster samples at 1:1000-10,000. J Biol Chem (2015) ncbi
Articles Reviewed
  1. Lattner J, Leng W, Knust E, Brankatschk M, Flores Benitez D. Crumbs organizes the transport machinery by regulating apical levels of PI(4,5)P2 in Drosophila. elife. 2019;8: pubmed publisher
  2. Ordonez D, Lee M, Feany M. α-synuclein Induces Mitochondrial Dysfunction through Spectrin and the Actin Cytoskeleton. Neuron. 2018;97:108-124.e6 pubmed publisher
  3. Flores Benitez D, Knust E. Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila. elife. 2015;4: pubmed publisher
  4. Chen H, Huang Y, Pfeiffer B, Yao X, Lee T. An enhanced gene targeting toolkit for Drosophila: Golic+. Genetics. 2015;199:683-94 pubmed publisher
  5. Furriols M, Casanova J. Germline and somatic vitelline proteins colocalize in aggregates in the follicular epithelium of Drosophila ovaries. Fly (Austin). 2014;8:113-9 pubmed publisher
  6. Chen D, Wu C, Zhao S, Geng Q, Gao Y, Li X, et al. Three RNA binding proteins form a complex to promote differentiation of germline stem cell lineage in Drosophila. PLoS Genet. 2014;10:e1004797 pubmed publisher
  7. Zacharogianni M, Aguilera Gomez A, Veenendaal T, Smout J, Rabouille C. A stress assembly that confers cell viability by preserving ERES components during amino-acid starvation. elife. 2014;3: pubmed publisher
  8. Khanna M, Mattie F, Browder K, Radyk M, Crilly S, Bakerink K, et al. Spectrin tetramer formation is not required for viable development in Drosophila. J Biol Chem. 2015;290:706-15 pubmed publisher