5-hydroxymethylcytosine Monoclonal Antibody (3I7)
Invitrogen
catalog: MA5-23525

quantity: 100µl
price: US 345
to the supplier

5-hydroxymethylcytosine Polyclonal Antibody
Invitrogen
catalog: PA5-40097

quantity: 50µg
price: US 345
to the supplier

5-Hydroxymethylcytosine Recombinant Rabbit Monoclonal Antibody (RM236)
Invitrogen
catalog: MA5-24695

quantity: 50µg
price: US 305
to the supplier

5-hmC 5-hydroxymethylcytosine (monoclonal)
Agrisera
catalog: AS16 3164

quantity: 100 µg
price:
to the supplier

5-hmC 5-hydroxymethylcytosine (polyclonal)
Agrisera
catalog: AS16 3166

quantity: 100 µl
price:
to the supplier

5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HM .
Epigentek
catalog: A-1018-010

citations: 1

quantity: 10 µg
price:
to the supplier

5-hmc Antibody (AP) LS-C250497
LifeSpan Biosciences
catalog: LS-C250497

total number of products: 7

quantity:
price:
to the supplier

5-hmc Antibody (APC) LS-C250499
LifeSpan Biosciences
catalog: LS-C250499

total number of products: 7

quantity:
price:
to the supplier

5-hmc Antibody (Biotin) LS-C250500
LifeSpan Biosciences
catalog: LS-C250500

total number of products: 7

quantity:
price:
to the supplier

5-hydroxymethylcytosine (5-hmC) monoclonal antibody, clone RM236
Abnova
catalog: MAB15116

quantity: 50 ug
price:
to the supplier

5-Hydroxymethylcytosine polyclonal antibody
Enzo Life Sciences
catalog: ADI-905-904-0050

total number of products: 4

Immunoprecipitation analysis: Hydroxymethylated DNA is efficiently enriched using the 5-Hydroxymethylcytosine pAb. DNA was immunoprecipitated from 1 ng of a mixed non-methylated/methylated/hydroxymethylated (10:1:1) DNA population. This population was comprised of a mixture of non-methylated plasmid DNA, a methylated version of the same plasmid containing a point mutation that introduces a BamHI restriction site, and a hydroxymethylated version of the same plasmid with a KpnI restriction site. After IP, the region of DNA containing the restriction site was amplified by PCR, digested with either BamHI (B) or KpnI (K), and visualized in a 1.4% (w/v) agarose/TAE/EtBr gel. The results indicate high sensitivity of 5-hydroxymethylcytosine pAb for 5-hydroxymethylcytosine DNA with no detectable crossreactivity to 5-methylcytosine DNA.

ELISA analysis: The 5-Hydroxymethylcytosine pAb demonstrates high sensitivity to 5-hydroxymethylcytosine in genomic DNA. In a standard ELISA workflow, 100 ng of purified genomic DNA from several murine tissue sources €” brain, kidney, liver, and thymus €” are coated per well. The 5-Hydroxymethylcytosine pAb and a secondary antibody (anti-rabbit horseradish peroxidase conjugate) are used at 1:1000 dilution in modified TBS and incubated at 37°C for 1 hour. Optical density was measured after 1 hour of ABTS substrate incubation at room temperature. Independent quantitation of 5-hydroxymethylcytosine levels using LC/MS analysis of genomic DNA from the same tissue sources indicates brain at 0.548%, kidney at 0.225%, liver at 0.107 %, and thymus at 0.030%, demonstrating high correlation with the colorimetric ELISA data.
quantity: 50 µg
price: 190 USD
to the supplier

5-Hydroxymethylcytosine polyclonal antibody
Enzo Life Sciences
catalog: ADI-905-904-0200

total number of products: 4

Immunoprecipitation analysis: Hydroxymethylated DNA is efficiently enriched using the 5-Hydroxymethylcytosine pAb. DNA was immunoprecipitated from 1 ng of a mixed non-methylated/methylated/hydroxymethylated (10:1:1) DNA population. This population was comprised of a mixture of non-methylated plasmid DNA, a methylated version of the same plasmid containing a point mutation that introduces a BamHI restriction site, and a hydroxymethylated version of the same plasmid with a KpnI restriction site. After IP, the region of DNA containing the restriction site was amplified by PCR, digested with either BamHI (B) or KpnI (K), and visualized in a 1.4% (w/v) agarose/TAE/EtBr gel. The results indicate high sensitivity of 5-hydroxymethylcytosine pAb for 5-hydroxymethylcytosine DNA with no detectable crossreactivity to 5-methylcytosine DNA.

ELISA analysis: The 5-Hydroxymethylcytosine pAb demonstrates high sensitivity to 5-hydroxymethylcytosine in genomic DNA. In a standard ELISA workflow, 100 ng of purified genomic DNA from several murine tissue sources €” brain, kidney, liver, and thymus €” are coated per well. The 5-Hydroxymethylcytosine pAb and a secondary antibody (anti-rabbit horseradish peroxidase conjugate) are used at 1:1000 dilution in modified TBS and incubated at 37°C for 1 hour. Optical density was measured after 1 hour of ABTS substrate incubation at room temperature. Independent quantitation of 5-hydroxymethylcytosine levels using LC/MS analysis of genomic DNA from the same tissue sources indicates brain at 0.548%, kidney at 0.225%, liver at 0.107 %, and thymus at 0.030%, demonstrating high correlation with the colorimetric ELISA data.
quantity: 200 µg
price: 592 USD
to the supplier

5-Hydroxymethylcytosine DNA ELISA kit
Enzo Life Sciences
catalog: ADI-900-225-0001

total number of products: 4

DNA Mass Spectroscopy versus ELISA analysis: The 5-hmC DNA ELISA Kit (prod. no. ADI-900-225) quantifies 5-hmC in numerous DNA samples with close correlation to LC-MS/MS-MRM analysis. DNA samples include: brain, kidney, liver, thymus, human embryonic stem cells and two carcinoma samples.

quantity: 96 wells
price: 463 USD
to the supplier

Anti-5-hydroxymethylcytosine (5-hmC) antibody [AB3/63.3] - ChIP Grade
Abcam
catalog: ab106918

citations: 6

quantity: 100 µg
price:
to the supplier

Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236]
Abcam
catalog: ab214728

quantity: 50 µg
price:
to the supplier

Anti-5-hydroxymethylcytosine (5-hmC) antibody
Abcam
catalog: ab231902

quantity: 100 µl
price:
to the supplier

5-Hydroxymethylcytosine (5-hmC) antibody (pAb)
Active Motif
catalog: 39769

citations: 18

quantity: 100 ul
price: 350 USD
to the supplier

5-Hydroxymethylcytosine (5-hmC) antibody (pAb)
Active Motif
catalog: 39791

citations: 9

quantity: 100 ug
price: 350 USD
to the supplier

5-Hydroxymethylcytosine (5-hmC) antibody (mAb)
Active Motif
catalog: 39999

citations: 4

quantity: 100 ug
price: 350 USD
to the supplier

Anti-5-Hydroxymethylcytosine [AB3/63.3]
Absolute Antibody
catalog: Ab00862-7.1

quantity: 200 μg
price: 350 USD
to the supplier

Anti-5-Hydroxymethylcytosine [AB3/63.3]
Absolute Antibody
catalog: Ab00862-7.4

quantity: 200 μg
price: 350 USD
to the supplier

Anti-5-Hydroxymethylcytosine [AB3/63.3]
Absolute Antibody
catalog: Ab00862-23.0

quantity: 200 μg
price: 350 USD
to the supplier

Imprint(R) Monoclonal Anti-5-hydroxymethylcytosine antibody produced in rat
MilliporeSigma
catalog: SAB4800002

quantity:
price:
to the supplier

Imprint(R) Monoclonal Anti-5-hydroxymethylcytosine antibody produced in mouse
MilliporeSigma
catalog: SAB4800049

quantity:
price:
to the supplier

5-hydroxymethylcytosine (5hmC) Antibody, clone AB3/63.3
EMD Millipore
catalog: MABE176

total number of products: 3

citations: 3

quantity: 100 μg
price:
to the supplier

Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3]
Ximbio
catalog: 152733

quantity:
price:
to the supplier