antibody

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14) [ICH1132UL] ichorbio

ICH1132UL

5 mg

312 GBP

monoclonal

rat

nonconjugated

RMP1-14

western blot, neutralization

ICH1132UL

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14) [ICH1132UL] ichorbio

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14) [ICH1132UL]

Bulk anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14)

ichorbio's anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. This means that ichorbio's ultra low-endoxin antibodies have the lowest endotoxin in the industry at less than 0.75 EU/mg. The purity of ichorbio's ultra low endotoxin antibodies are unsurpassed at >98% and they are all pathogen tested and third-party certified by IDEXX BioReseach of meet the lowest mycoplasma specification and free of any viral pathogens of concern. ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.

PD-1

RMP1-14

5 mg

312 GBP

25mg

832

50mg

1352

100mg

2080

Rat IgG2a

Programmed cell death protein 1, Pdcd1, CD279

Q02242

Rat

Mouse

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14) recognizes an epitope on Mouse PD-1

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Subset of double negative thymocytes, activated T and B cells

CD279, also known as programmed death-1 (PD-1) is a 50-55 kD immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. This RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production.

Mouse PD-1 transfected BHK cells

≥ 2.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>98% by SDS-PAGE and HPLC

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 1%

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: Mycoplasma spp Mycoplasma pulmonis Pneumonia virus of mice Kilham's rat virus Toolan's H1 virus Rat parvovirus Lymphocytic choriomeningitis virus Rat cytomegalovirus Sendai virus Rat coronavirus Sialodacryoadenitis virus Rat minute virus Seoul virus Mouse adenovirus Reovirus 3 Rat theilovirus

anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Western Blot, Blocking, Functional Assays

Blocking: Clone RMP1-14 antibody has been shown to block the binding of both mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. For this anti-PD-1 (clone RMP1-14), we suggest to inject 200 ug/mouse ip generally on days 3, 6 and 9, post injection of tumor. Sometime up to six injections depending on the length of the experiment may be required. Each investigator should determine their own optimal working dilution for specific applications.

Rat IgG2a In Vivo Isotype Control - Ultra Low Endotoxin [1-1] (ICH2244UL)

Anti-PD-1 In Vivo Antibody - Low Endotoxin [RMP1-14] (ICH1132)

Anti-PD-1 In Vivo Antibody - Low Endotoxin [J43] (ICH1116) , Anti-PD-1 In Vivo Antibody - Low Endotoxin [29F.1A12] (ICH1091) , Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin [29F.1A12] (ICH1091UL)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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