Anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) [ICH1132] ichorbio

product summary

company name :

ichorbio

product type :

antibody

product name :

Anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) [ICH1132] ichorbio

catalog :

ICH1132

quantity :

1 mg

price :

80 GBP

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

RMP1-14

reactivity :

mouse

application :

western blot, neutralization

more info or order :

ichorbio product webpage

product information

Product Type :

Low Endotoxin

ichorbio Cat Number :

ICH1132

Page Title :

Anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) [ICH1132] ichorbio

Page Heading :

Anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) [ICH1132]

Page Heading :

Bulk anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14)

Product Benefits :

ichorbio's anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin ( here for more information. ichorbio: the best antibodies for in vivo research.

Target :

PD-1

Clone :

RMP1-14

Size1 :

1 mg

Academic Price1 :

80 GBP

Size2 :

5mg

Academic Price2 :

240

Size3 :

25mg

Academic Price3 :

640

Size4 :

50mg

Academic Price4 :

1040

Size5 :

100mg

Academic Price5 :

1600

Isotype :

Rat IgG2a

Other names :

Programmed cell death protein 1, Pdcd1, CD279

UniProt :

Q02242

Host :

Rat

Species Reactivity :

Mouse

Specificity :

Anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) recognizes an epitope on Mouse PD-1

Purification method :

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution :

Subset of double negative thymocytes, activated T and B cells

Background :

CD279, also known as programmed death-1 (PD-1) is a 50-55 kD immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. This RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production.

Immunogen :

Mouse PD-1 transfected BHK cells

Concentration :

1.0 - 5.0 mg/ml

Formulation :

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity :

>95% by SDS-PAGE and HPLC

Endotoxin :

≤ 1.0 EU/mg as determined by the LAL method

Aggregation :

Aggregation level ≤ 5%

Storage :

anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) is stable when stored at 2-8°C. For longer term storage (> 3 months) it is recommended to store this antibody at -70oC.

Applications :

Western Blot, Blocking

Application Notes :

Blocking: Clone RMP1-14 antibody has been shown to block the binding of both mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. For this anti-PD-1 (clone RMP1-14), we suggest to inject 200 ug/mouse ip generally on days 3, 6 and 9, post injection of tumor. Sometime up to six injections depending on the length of the experiment may be required. Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control :

Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)

Related Antibody :

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin [RMP1-14] (ICH1132UL)

Antibodies against the same target :

Anti-PD-1 In Vivo Antibody - Low Endotoxin [J43] (ICH1116) , Anti-PD-1 In Vivo Antibody - Low Endotoxin [29F.1A12] (ICH1091) , Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin [29F.1A12] (ICH1091UL)

Use :

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Pricing Note :

Contact for industrial pricing

more info or order :

ichorbio product webpage