antibody

Anti-IFNAR1 In Vivo Antibody - Low Endotoxin (MAR1-5A3) [ICH1122] ichorbio

ICH1122

1 mg

80 GBP

monoclonal

mouse

nonconjugated

MAR1-5A3

western blot, neutralization, immunoprecipitation

ICH1122

Anti-IFNAR1 In Vivo Antibody - Low Endotoxin (MAR1-5A3) [ICH1122] ichorbio

Anti-IFNAR1 In Vivo Antibody - Low Endotoxin (MAR1-5A3) [ICH1122]

Bulk anti-IFNAR1 In Vivo Antibody - Low Endotoxin (MAR1-5A3)

ichorbio's anti-IFNAR1 In Vivo Antibody - Low Endotoxin (MAR1-5A3) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin ( here for more information. ichorbio: the best antibodies for in vivo research.

IFNAR1

MAR1-5A3

1 mg

80 GBP

5mg

240

25mg

640

50mg

1040

100mg

1600

Mouse IgG1

Interferon alpha/beta receptor 1, IFN-R-1, IFN-alpha/beta receptor 1, Type I interferon receptor 1, Ifar, Ifnar

P33896

Mouse

Mouse

Anti-IFNAR1 In Vivo Antibody - Low Endotoxin (MAR1-5A3) recognizes the extracellular domain of the IFNAR1 subunit of the mouse IFN-alpha / beta receptor.

IFNAR1 and IFNAR2 are coexpressed on nearly all cells.

The antibody when prepared specifically for In vivo functional assays blocks type I IFN receptor signaling both In vitro and In vivo without depleting IFNAR1 bearing cells. This antibody was produced by In vivo genetic immunization of IFNAR1 knockout mice with a plasmid encoding the extracellular domain of murine IFNAR1. IFNAR1 and IFNAR2 are coexpressed on nearly all cells and make up the heterodimeric receptor that binds all type I IFNs (IFN alpha and beta). Type I IFNs are a family of cytokines that have been shown to promote anti-viral, anti-microbial, anti-tumor and autoimmune responses In vivo.

This antibody was produced by In vivo genetic immunization of IFNAR1 knockout mice with a plasmid encoding the extracellular domain of murine IFNAR1.

≥ 2.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

anti-IFNAR1 In Vivo Antibody - Low Endotoxin (MAR1-5A3) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. NOTE: Do not freeze as this antibody at -20°C as this clone will sometimes precipitate over long period of time.

Immunoprecipitation, Western Blot, Blocking

Blocking: Clone MAR1-5A3 has a short half-life, basically because every cell expresses the IFNAR1 receptor and the receptor recycles very rapidly. And, if you want to block function in vivo, you need to be sure that all of the receptors are blocked continually in all compartments. Therefore, you need a large in vivo loading dose (2.5 mg/mouse) to saturate all the binding sites in vivo and then maintain a high enough level to keep them saturated. For in vivo blocking studies we recommend give a loading dose of 2.5 mg/mouse and follow with a weekly dose of 0.5 mg/mouse. The half-live following a 2.5 mg loading dose is about 5 days. [However, if you only inject a low dose of 250 micrograms, then the half life is 1.5 days – because you haven’t saturated the mouse]. Each investigator should determine their own optimal working dilution for specific applications.

Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247)

Anti-IFNAR1 In Vivo Antibody - Ultra Low Endotoxin [MAR1-5A3] (ICH1122UL)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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