antibody

Anti-IFN beta In Vivo Antibody - Ultra Low Endotoxin (HDB-4A7) [ICH1110UL] ichorbio

ICH1110UL

5 mg

312 GBP

monoclonal

mouse

nonconjugated

HDB-4A7

western blot, neutralization

ICH1110UL

Anti-IFN beta In Vivo Antibody - Ultra Low Endotoxin (HDB-4A7) [ICH1110UL] ichorbio

Anti-IFN beta In Vivo Antibody - Ultra Low Endotoxin (HDB-4A7) [ICH1110UL]

Bulk anti-IFN beta In Vivo Antibody - Ultra Low Endotoxin (HDB-4A7)

ichorbio's anti-IFN beta In Vivo Antibody - Ultra Low Endotoxin (HDB-4A7) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. This means that ichorbio's ultra low-endoxin antibodies have the lowest endotoxin in the industry at less than 0.75 EU/mg. The purity of ichorbio's ultra low endotoxin antibodies are unsurpassed at >98% and they are all pathogen tested and third-party certified by IDEXX BioReseach of meet the lowest mycoplasma specification and free of any viral pathogens of concern. ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.

IFN beta

HDB-4A7

5 mg

312 GBP

25mg

832

50mg

1352

100mg

2080

Mouse IgG2a

IFN-b, IFN-beta, Type-1 interferon, Interferon beta

Mouse

Mouse

Anti-IFN beta In Vivo Antibody - Ultra Low Endotoxin (HDB-4A7) recognizes an epitope on Mouse IFN-beta and binds specifically to murine IFNbeta and does not bind murine IFNg or several different IFNa species (a-1,4,5 or 13); HDb-4A7 binds both recombinant and natural forms

This antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Plasmid DNA encoding murine IFNb

1.0 - 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>98% by SDS-PAGE and HPLC

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 1%

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: Mycoplasma spp. Mycoplasma pulmonis Sendai virus Mouse hepatitis virus Pneumonia virus of mice Minute virus of mice Mouse parvovirus (MPV1-5) Theiler's murine encephalomyelitis virus Murine norovirus Reovirus 3 Mouse rotavirus Ectromelia virus Lymphocytic choriomeningitis virus Polyoma virus Lactate dehydrogenase-elevating virus Mouse adenovirus (MAD1, MAD2) Mouse cytomegalovirus K virus Mouse thymic virus Hantaan virus Corynebacterium bovis Corynebacterium spp. (HAC2)

anti-IFN beta In Vivo Antibody - Ultra Low Endotoxin (HDB-4A7) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Western Blot, Blocking, Functional Assays

Functional Assay: In Vitro Activity of HDB-4A7 - inhibits IFNb-induced Stat1 phosphorylation (level of inhibition is similar to that seen using anti-IFNAR1 mAb (MAR1-5A3). - blocks IFNb induction of MHC-I expression (H2-Kb) using L929 or fibrosarcoma cell lines. - neutralizes IFNb-induced antiviral activity in vitro following infection of L929 cells with VSV In Vivo Activity of HDB-4A7 - circulates with a half-life of ~ 14 days. - blocks in vivo IFNb leading to increased the lethality of mice infected with West Nile Virus (WNV), similar to susceptibility of IFNb-/- mice. Each investigator should determine their own optimal working dilution for specific applications.

Mouse IgG2a In Vivo Isotype Control - Ultra Low Endotoxin [C1.18.4] (ICH2245UL)

Anti-IFN beta In Vivo Antibody - Low Endotoxin [HDB-4A7] (ICH1110)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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