Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) [ICH1091UL] ichorbio

product summary

company name :

ichorbio

product type :

antibody

product name :

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) [ICH1091UL] ichorbio

catalog :

ICH1091UL

quantity :

5 mg

price :

312 GBP

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

29F.1A12

reactivity :

mouse

application :

western blot, neutralization, flow cytometry

more info or order :

ichorbio product webpage

product information

Product Type :

Ultra Low Endotoxin

ichorbio Cat Number :

ICH1091UL

Page Title :

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) [ICH1091UL] ichorbio

Page Heading :

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) [ICH1091UL]

Page Heading :

Bulk anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12)

Product Benefits :

ichorbio's anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. This means that ichorbio's ultra low-endoxin antibodies have the lowest endotoxin in the industry at less than 0.75 EU/mg. The purity of ichorbio's ultra low endotoxin antibodies are unsurpassed at >98% and they are all pathogen tested and third-party certified by IDEXX BioReseach of meet the lowest mycoplasma specification and free of any viral pathogens of concern. ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.

Target :

PD-1

Clone :

29F.1A12

Size1 :

5 mg

Academic Price1 :

312 GBP

Size2 :

25mg

Academic Price2 :

832

Size3 :

50mg

Academic Price3 :

1352

Size4 :

100mg

Academic Price4 :

2080

Isotype :

Rat IgG2a

Other names :

Programmed cell death protein 1, Pdcd1, CD279

UniProt :

Q02242

Host :

Rat

Species Reactivity :

Mouse

Specificity :

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) recognizes an epitope on Mouse PD-1. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

Purification method :

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution :

Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.Subset of double negative thymocytes, activated T and B cells

Background :

Programmed death-1 (PD-1), also know as CD279 is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Like the clones RMP1-14 and J43 antibodies, the 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo.

Immunogen :

PD-1 cDNA followed by PD-1-Ig fusion protein

Concentration :

≥ 2.0 mg/ml

Formulation :

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity :

>98% by SDS-PAGE and HPLC

Endotoxin :

≤ 0.75 EU/mg as determined by the LAL method

Aggregation :

Aggregation level ≤ 1%

IMPACT Pathogen Test :

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: Mycoplasma spp Mycoplasma pulmonis Pneumonia virus of mice Kilham's rat virus Toolan's H1 virus Rat parvovirus Lymphocytic choriomeningitis virus Rat cytomegalovirus Sendai virus Rat coronavirus Sialodacryoadenitis virus Rat minute virus Seoul virus Mouse adenovirus Reovirus 3 Rat theilovirus

Storage :

anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Applications :

Flow Cytometry, Western Blot, Blocking

Application Notes :

Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml. Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3,6,9 @ 200 ug per animal per injection) then the tumor will regress, and disappear. Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control :

Rat IgG2a In Vivo Isotype Control - Ultra Low Endotoxin [1-1] (ICH2244UL)

Related Antibody :

Anti-PD-1 In Vivo Antibody - Low Endotoxin [29F.1A12] (ICH1091)

Antibodies against the same target :

Anti-PD-1 In Vivo Antibody - Low Endotoxin [J43] (ICH1116) , Anti-PD-1 In Vivo Antibody - Low Endotoxin [RMP1-14] (ICH1132) , Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin [RMP1-14] (ICH1132UL)

Use :

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Pricing Note :

Contact for industrial pricing

more info or order :

ichorbio product webpage