Anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) [ICH1091] ichorbio

product summary

company name :

ichorbio

product type :

antibody

product name :

Anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) [ICH1091] ichorbio

catalog :

ICH1091

quantity :

1 mg

price :

80 GBP

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

29F.1A12

reactivity :

mouse

application :

western blot, neutralization, flow cytometry

more info or order :

ichorbio product webpage

product information

Product Type :

Low Endotoxin

ichorbio Cat Number :

ICH1091

Page Title :

Anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) [ICH1091] ichorbio

Page Heading :

Anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) [ICH1091]

Page Heading :

Bulk anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12)

Product Benefits :

ichorbio's anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin ( here for more information. ichorbio: the best antibodies for in vivo research.

Target :

PD-1

Clone :

29F.1A12

Size1 :

1 mg

Academic Price1 :

80 GBP

Size2 :

5mg

Academic Price2 :

240

Size3 :

25mg

Academic Price3 :

640

Size4 :

50mg

Academic Price4 :

1040

Size5 :

100mg

Academic Price5 :

1600

Isotype :

Rat IgG2a

Other names :

Programmed cell death protein 1, Pdcd1, CD279

UniProt :

Q02242

Host :

Rat

Species Reactivity :

Mouse

Specificity :

Anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) recognizes an epitope on Mouse PD-1. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

Purification method :

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution :

Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.Subset of double negative thymocytes, activated T and B cells

Background :

Programmed death-1 (PD-1), also know as CD279 is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Like the clones RMP1-14 and J43 antibodies, the 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo.

Immunogen :

PD-1 cDNA followed by PD-1-Ig fusion protein

Concentration :

1.0 - 5.0 mg/ml

Formulation :

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity :

>95% by SDS-PAGE and HPLC

Endotoxin :

≤ 1.0 EU/mg as determined by the LAL method

Aggregation :

Aggregation level ≤ 5%

Storage :

anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Applications :

Flow Cytometry, Western Blot, Blocking

Application Notes :

Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml. Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3,6,9 @ 200 ug per animal per injection) then the tumor will regress, and disappear. Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control :

Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)

Related Antibody :

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin [29F.1A12] (ICH1091UL)

Antibodies against the same target :

Anti-PD-1 In Vivo Antibody - Low Endotoxin [J43] (ICH1116) , Anti-PD-1 In Vivo Antibody - Low Endotoxin [RMP1-14] (ICH1132) , Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin [RMP1-14] (ICH1132UL)

Use :

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Pricing Note :

Contact for industrial pricing

more info or order :

ichorbio product webpage