Anti-CD28 In Vivo Antibody - Ultra Low Endotoxin (37.51) [ICH1055UL] ichorbio

product summary

company name :

ichorbio

product type :

antibody

product name :

Anti-CD28 In Vivo Antibody - Ultra Low Endotoxin (37.51) [ICH1055UL] ichorbio

catalog :

ICH1055UL

quantity :

5 mg

price :

312 GBP

clonality :

monoclonal

host :

golden hamster

conjugate :

nonconjugated

clone name :

37.51

application :

western blot, neutralization, immunoprecipitation

more info or order :

ichorbio product webpage

product information

Product Type :

Ultra Low Endotoxin

ichorbio Cat Number :

ICH1055UL

Page Title :

Anti-CD28 In Vivo Antibody - Ultra Low Endotoxin (37.51) [ICH1055UL] ichorbio

Page Heading :

Anti-CD28 In Vivo Antibody - Ultra Low Endotoxin (37.51) [ICH1055UL]

Page Heading :

Bulk anti-CD28 In Vivo Antibody - Ultra Low Endotoxin (37.51)

Product Benefits :

ichorbio's anti-CD28 In Vivo Antibody - Ultra Low Endotoxin (37.51) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. This means that ichorbio's ultra low-endoxin antibodies have the lowest endotoxin in the industry at less than 0.75 EU/mg. The purity of ichorbio's ultra low endotoxin antibodies are unsurpassed at >98% and they are all pathogen tested and third-party certified by IDEXX BioReseach of meet the lowest mycoplasma specification and free of any viral pathogens of concern. ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.

Target :

CD28

Clone :

37.51

Size1 :

5 mg

Academic Price1 :

312 GBP

Size2 :

25mg

Academic Price2 :

832

Size3 :

50mg

Academic Price3 :

1352

Size4 :

100mg

Academic Price4 :

2080

Isotype :

Syrian Hamster IgG

Host :

Syrian Hamster

Species Reactivity :

Mouse

Specificity :

Anti-CD28 In Vivo Antibody - Ultra Low Endotoxin (37.51) recognizes the CD28 co-stimulatory receptor, a ligand of CD80 (B7-1) and CD86 (B7-2). CD28 is important for transmitting co-stimulatory signals for optimal T-cell activation.

Antigen Distribution :

Anti-Mouse CD28 recognizes the CD28 co-stimulatory receptor, a ligand of CD80 (B7-1) and CD86 (B7-2). CD28 is important for transmitting co-stimulatory signals for optimal T-cell activation.

Background :

CD28 (Cluster of Differentiation 28) is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. It is expressed on the cell surface as disulfide-linked homodimer or as monomer. CD28 is the receptor for B7.1 (CD80) and B7.2 (CD86). When activated by Toll-like receptor ligands, the B7.1 expression is upregulated in antigen presenting cells (APCs). The B7.2 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. Stimulation through CD28 in addition to the TCR can provide a potent co-stimulatory signal to T cells for the production of various interleukins (IL-2 and IL-6 in particular). CD28 possesses an intracellular domain with several residues that are critical for its effective signalling. CD28 also contains two proline-rich motifs that are able to bind SH3-containing proteins. CD28 has also been found to stimulate eosinophil granulocytes where its ligation with anti-CD28 leads to the release of IL-2, IL4, IL-13 and IFN-γ.

Immunogen :

C57BL/6 mouse T-cell lymphoma EL-4

Concentration :

1.0 - 5.0 mg/ml

Formulation :

20 mM Histidine pH 6.0 containing 150 mM NaCl. NOTE: A buffer exchange into PBS for conjugation and MES for functional assays may be required depending on your application.

Purity :

>98% by SDS-PAGE and HPLC

Endotoxin :

≤ 0.75 EU/mg as determined by the LAL method

Aggregation :

Aggregation level ≤ 1%

IMPACT Pathogen Test :

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for: Mycoplasma spp Mycoplasma pulmonis Pneumonia virus of mice Kilham's rat virus Toolan's H1 virus Hamster parvovirus Lymphocytic choriomeningitis Minute virus of mice Theiler's murine encephalomyelitis virus Sendai virus Reovirus 3 Hantaan virus

Storage :

anti-CD28 In Vivo Antibody - Ultra Low Endotoxin (37.51) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Applications :

Immunoprecipitation, Western Blot, Blocking, Functional Assays

Application Notes :

Functional Assay: Anti-Mouse CD28 (clone 37.51) has been reported in costimulation of T cells In vitro and In vivo. Each investigator should determine their own optimal working dilution for specific applications.

Related Antibody :

Anti-CD28 In Vivo Antibody - Low Endotoxin [37.51] (ICH1055)

Antibodies against the same target :

Anti-CD28 In Vivo Antibody - Low Endotoxin [CD28.2] (ICH1013)

Use :

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Pricing Note :

Contact for industrial pricing

more info or order :

ichorbio product webpage