antibody

Anti-CD28 In Vivo Antibody - Low Endotoxin (CD28.2) [ICH1013] ichorbio

ICH1013

0.5 mg

160 GBP

monoclonal

mouse

nonconjugated

CD28.2

immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

ICH1013

Anti-CD28 In Vivo Antibody - Low Endotoxin (CD28.2) [ICH1013] ichorbio

Anti-CD28 In Vivo Antibody - Low Endotoxin (CD28.2) [ICH1013]

Bulk anti-CD28 In Vivo Antibody - Low Endotoxin (CD28.2)

ichorbio's anti-CD28 In Vivo Antibody - Low Endotoxin (CD28.2) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin ( here for more information. ichorbio: the best antibodies for in vivo research.

CD28

CD28.2

0.5 mg

160 GBP

1mg

280

Mouse IgG1

TP44

P10747

Mouse

Human

Anti-CD28 In Vivo Antibody - Low Endotoxin (CD28.2) recognizes an epitope on Human CD28

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells

CD28 (Cluster of Differentiation 28) is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. It is expressed on the cell surface as disulfide-linked homodimer or as monomer. CD28 is the receptor for B7.1 (CD80) and B7.2 (CD86). When activated by Toll-like receptor ligands, the B7.1 expression is upregulated in antigen presenting cells (APCs). The B7.2 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. Stimulation through CD28 in addition to the TCR can provide a potent co-stimulatory signal to T cells for the production of various interleukins (IL-2 and IL-6 in particular). CD28 possesses an intracellular domain with several residues that are critical for its effective signalling. CD28 also contains two proline-rich motifs that are able to bind SH3-containing proteins. CD28 has also been found to stimulate eosinophil granulocytes where its ligation with anti-CD28 leads to the release of IL-2, IL4, IL-13 and IFN-gamma

1.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>90% by SDS-PAGE and HPLC

<0.1 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

anti-CD28 In Vivo Antibody - Low Endotoxin (CD28.2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

IHC (Frozen), Flow Cytometry, Immunoprecipitation

Each investigator should determine their own optimal working dilution for specific applications.

Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247)

Anti-CD28 In Vivo Antibody - Ultra Low Endotoxin [37.51] (ICH1055UL) , Anti-CD28 In Vivo Antibody - Low Endotoxin [37.51] (ICH1055)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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