Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) [ICH1010] ichorbio

product summary

company name :

ichorbio

product type :

antibody

product name :

Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) [ICH1010] ichorbio

catalog :

ICH1010

quantity :

1 mg

price :

80 GBP

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3G8

reactivity :

human

application :

immunohistochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

more info or order :

ichorbio product webpage

product information

Product Type :

Low Endotoxin

ichorbio Cat Number :

ICH1010

Page Title :

Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) [ICH1010] ichorbio

Page Heading :

Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) [ICH1010]

Page Heading :

Bulk anti-CD16 In Vivo Antibody - Low Endotoxin (3G8)

Product Benefits :

ichorbio's anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin ( here for more information. ichorbio: the best antibodies for in vivo research.

Target :

CD16

Clone :

3G8

Size1 :

1 mg

Academic Price1 :

80 GBP

Size2 :

5mg

Academic Price2 :

240

Size3 :

25mg

Academic Price3 :

640

Size4 :

50mg

Academic Price4 :

1040

Size5 :

100mg

Academic Price5 :

1600

Isotype :

Mouse IgG1

Other names :

FCGR3A, Fc-gamma RIII, FcRIII

UniProt :

P08637

Host :

Mouse

Species Reactivity :

Human

Specificity :

Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) recognizes an epitope on Human CD16

Purification method :

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution :

CD16 is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans.

Background :

CD16 is known as low affinity IgG receptor III (Fc gamma RIII) and is expressed as two distinct forms (CD16a and CD16b). CD16a (Fc gamma RIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (Fc gamma RIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils.

Immunogen :

Human PMN cells

Concentration :

≥ 2.0 mg/ml

Formulation :

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity :

>95% by SDS-PAGE and HPLC

Endotoxin :

≤ 1.0 EU/mg as determined by the LAL method

Aggregation :

Aggregation level ≤ 5%

Storage :

anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) is stable when stored at 2-8°C.

Applications :

IHC (Frozen), Flow Cytometry, Immunoprecipitation, Blocking

Application Notes :

Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. Blocking: Clone 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation. Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control :

Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247)

Antibodies against the same target :

Anti-CD32/CD16 In Vivo Antibody - Low Endotoxin [2.4G2] (ICH1056)

Use :

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Pricing Note :

Contact for industrial pricing

more info or order :

ichorbio product webpage