Anti-CD3 In Vivo Antibody - Low Endotoxin (HIT3a) [ICH1003] ichorbio

product summary

company name :

ichorbio

product type :

antibody

product name :

Anti-CD3 In Vivo Antibody - Low Endotoxin (HIT3a) [ICH1003] ichorbio

catalog :

ICH1003

quantity :

0.5 mg

price :

280 GBP

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HIT3a

reactivity :

human

more info or order :

ichorbio product webpage

product information

Product Type :

Low Endotoxin

ichorbio Cat Number :

ICH1003

Page Title :

Anti-CD3 In Vivo Antibody - Low Endotoxin (HIT3a) [ICH1003] ichorbio

Page Heading :

Anti-CD3 In Vivo Antibody - Low Endotoxin (HIT3a) [ICH1003]

Page Heading :

Bulk anti-CD3 In Vivo Antibody - Low Endotoxin (HIT3a)

Product Benefits :

ichorbio's anti-CD3 In Vivo Antibody - Low Endotoxin (HIT3a) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin ( here for more information. ichorbio: the best antibodies for in vivo research.

Target :

CD3

Clone :

HIT3a

Size1 :

0.5 mg

Academic Price1 :

280 GBP

Isotype :

Mouse IgG2a

Other names :

Host :

Mouse

Species Reactivity :

Human

Specificity :

Anti-CD3 In Vivo Antibody - Low Endotoxin (HIT3a) recognizes an epitope on Human CD3

Purification method :

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution :

The CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-ALL Cells.

Background :

Anti-CD3 may be used for enumerating immunocompetent T-lymphocytes in peripheral blood. Clone UCHT-1 is also useful in histology for localization of T-lymphocytes in tissue and may be used to enrich T-cells by cell sorting. This T-cell marker has also been used to determine T-versus B-cell lymphomas and leukemias.

Concentration :

1.0 mg/ml

Formulation :

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity :

>95% by SDS-PAGE and analyzed by silver stain.

Endotoxin :

<0.1 EU/mg as determined by the LAL method

Aggregation :

Aggregation level ≤ 5%

Storage :

anti-CD3 In Vivo Antibody - Low Endotoxin (HIT3a) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Applications :

Functional Assays

Application Notes :

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control :

Mouse IgG2a In Vivo Isotype Control - Low Endotoxin [C1.18.4] (ICH2245)

Antibodies against the same target :

Anti-CD3 In Vivo Antibody - Low Endotoxin [UCHT-1] (ICH1002) , Anti-CD3 In Vivo Antibody - Low Endotoxin [17A2] (ICH1039)

Use :

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Pricing Note :

Contact for industrial pricing

more info or order :

ichorbio product webpage