Anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) [ICH1002] ichorbio

product summary

company name :

ichorbio

product type :

antibody

product name :

Anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) [ICH1002] ichorbio

catalog :

ICH1002

quantity :

1 mg

price :

80 GBP

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

UCHT-1

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

ichorbio product webpage

product information

Product Type :

Low Endotoxin

ichorbio Cat Number :

ICH1002

Page Title :

Anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) [ICH1002] ichorbio

Page Heading :

Anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) [ICH1002]

Page Heading :

Bulk anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1)

Product Benefits :

ichorbio's anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin ( here for more information. ichorbio: the best antibodies for in vivo research.

Target :

CD3

Clone :

UCHT-1

Size1 :

1 mg

Academic Price1 :

80 GBP

Size2 :

5mg

Academic Price2 :

240

Size3 :

25mg

Academic Price3 :

640

Size4 :

50mg

Academic Price4 :

1040

Size5 :

100mg

Academic Price5 :

1600

Isotype :

Mouse IgG1

Other names :

CD3 epsilon chain, CD3E, T-cell surface antigen T3/Leu-4 epsilon chain

UniProt :

P07766

Host :

Mouse

Species Reactivity :

Human

Specificity :

Anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) recognizes Human CD3. anti-Human CD3 recognizes a (Mr 22-28 kDa) T-cell surface glycoprotein. The epitope recognized by the CD3 antibody is expressed on a constant region of the epsilon chain of the CD3 antigen/T-cell receptor complex (TCR)

Purification method :

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution :

The CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-ALL Cells.

Background :

Anti-CD3 may be used for enumerating immunocompetent T-lymphocytes in peripheral blood. Clone UCHT-1 is also useful in histology for localization of T-lymphocytes in tissue and may be used to enrich T-cells by cell sorting. This T-cell marker has also been used to determine T-versus B-cell lymphomas and leukemias. The UCHT-1 clone is recognized in the human leukocyte differentiation antigen workshop III 471.

Immunogen :

Unknown

Concentration :

≥ 2.0 mg/ml

Formulation :

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity :

>95% by SDS-PAGE and HPLC

Endotoxin :

<1.0 EU/mg as determined by the LAL method

Aggregation :

Aggregation level ≤ 5%

Storage :

anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Applications :

Flow Cytometry, Immunoprecipitation, Western Blot

Application Notes :

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3. A suggested method would be to stain cells expressing CD3 with 2.0 µg per million cells in a total staining volume of 100 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin. Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control :

Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247)

Antibodies against the same target :

Anti-CD3 In Vivo Antibody - Low Endotoxin [HIT3a] (ICH1003) , Anti-CD3 In Vivo Antibody - Low Endotoxin [17A2] (ICH1039)

Use :

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Pricing Note :

Contact for industrial pricing

more info or order :

ichorbio product webpage