antibody

Anti-p53 [PAb242]

153297

monoclonal

mouse

nonconjugated

22059

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

153297

ELISA IHC IF IP WB

p53

Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, p53, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53

Mouse

p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. PAB 1802 may be used to show the expression of human p53 cDNA in cos-l cells.

Mouse

SV40-transformed cell line BALB/c SVA31 E7

IgG1

MEFs

53

Sp2/0-Ag14

Cells were grown at 37°C and at 10% (vol/vol) CO2 in air. The cell culture medium was Dulbecco modified Eagle minimum essential medium supplemented with 10% (vol/vol) fetal calf serumplus 500 U of penicillin and 100ug Streptomycin per ml.

Balb/c

Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, DNA Damage and Repair, Epigenetics & Nuclear Signalling

This monoclonal works well on paraffin sections of mouse tissues. The epitope of has been mapped to the sequence 18-TFSGLWKLLP-27 in the N-terminal domain of mouse p53 (Yewdell et al., 1986; Lane et al., 1996).