antibody

Anti-BORIS [20B11]

152812

monoclonal

mouse

nonconjugated

140690

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

152812

ChIP ELISA IHC IF IP WB

BORIS

Brother of the regulator of imprinted sites, Cancer/testis antigen 27, CCCTC binding factor (zinc finger protein) like, CCCTC-binding factor, CT27, CTCF paralog, CTCF T, CTCF-like protein, Ctcfl, CTCFL_HUMAN, dJ579F20.2, HMG 1L1, HMGB1L1, MGC163358, MGC169105, MGC169106

Human

Reagent for research, diagnostic tool. BORIS protein has been identified as Cancer-Testis Antigen (CTA) with testis-specific paralogue of the CCCTC-binding factor. Recent studies have demonstrated that d BORIS is directly responsible for the transcriptional activation of TSP50 (testes-specific protease 50).

Mouse

Synthetic peptide within the BORIS C-terminal domain (aa 614-648) (CG)GEMFPVACRETTARVKEE (NB - the first two aa do not belong to BORIS)

IgG3 kappa

MCF7 cell lysates

83

Vials with hybridoma cell from the liquid nitrogen storage should be rapidly thawed. After thawing, the cell suspension needs to be added slowly using a sterile pipette into 10ml of warmed HM20 medium, the suspended cells centrifuged at 1200 rpm for 10 min and the supernatant discarded. The cell pellet should be gently re-suspended in 9ml of HM20-10% HCF (Hybridoma Cloning Factor, BioVeris Corporation) and transferred into a small flask (T25). Cells should be incubated at 37°C and 10% CO2 and passaged when they were 90% confluent. Cells should be successively grown in the medium with gradually decreasing amounts of HCF from 10% to 5% to 2.5% and finally without any HCF. Depending upon the confluency of the culture, cells should be transferred from the small flask to a medium sized flask (T75) during one of these stages.

Cancer, Epigenetics & Nuclear Signalling