Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3] | Ximbio 152733 product information

product summary

company name :

Ximbio

product type :

antibody

product name :

Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3]

catalog :

152733

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

AB3/63.3

application :

immunocytochemistry, immunoprecipitation

more info or order :

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product information

Name :

Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3]

Catalogue Number :

152733

Applications :

IF IP DB

Antigen/Gene or Protein Targets :

5-Hydroxymethylcytosine (5hmC)

Reactivity :

All

Relevance :

The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethylcytosine may regulate gene expression or initiate DNA demethylation.

Host :

Rat

Immunogen :

5-Hydroxymethylcytosine conjugated to BSA

Subclass :

IgG2a kappa

Positive Control :

Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA

Research Area :

DNA Damage and Repair, Epigenetics & Nuclear Signalling, Gene Expression, Stem Cell Biology

Notes :

Evaluated by Dot Blot in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA. Dot Blot Analysis: 10 µg/mL and 1 µg/mL of this antibody detected 5-hydroxymethylcytosine in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA. Non-species specific antibody against modified DNA. Expected to detect 5-hydroxymethylcytosine in all species.

more info or order :

Ximbio product webpage