product summary
company name :
Ximbio
product type :
antibody
product name :
Anti-5T4 [B5C9]
catalog :
152710
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
12509
reactivity :
mouse
application :
western blot, ELISA, immunocytochemistry, FACS
more info or order :
Ximbio product webpage
product information
Name :
Anti-5T4 [B5C9]
Catalogue Number :
152710
Applications :
ELISA FACS IF Fn WB
Antigen/Gene or Protein Targets :
5T4 oncofetal glycoprotein
Synonyms :
Trophoblast Glycoprotein; 5T4 Oncofetal Trophoblast Glycoprotein; Wnt-Activated Inhibitory Factor 1; 5T4 Oncotrophoblast Glycoprotein; 5T4 Oncofetal Antigen; WAIF1; M6P1; 5T4; 5T4AG
Reactivity :
Mouse
Relevance :
The murine 5T4 monoclonal antibody, clone B5C9, defines the human 5T4 oncofoetal antigen, a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development.
Host :
Mouse
Subclass :
IgG1
Molecular Weight (kDa) :
46.5
Recommended Growing Conditions :
DMEM supplemented with heat inactivated 10% fetal calf serum, 2 mM L-glutamine, 100 units/ml penicillin and 100 µg/ml streptomycin solution and maintained at 37°C in a humidified atmosphere of 5% CO2/95% air.
Strain :
C57BL/6
Research Area :
Cancer, Stem Cell Biology
Notes :
Clone B5C9 inhibits the chemotactic migration towards CXCL12 exhibited by differentiating WT-ES cells (to a lesser extent than clone B1C3). IC50 for the maximally inhibitory mAb B5C9 was 6.8 µg±1.5. Epitope: proximal LRR domain of m5T4.
more info or order :
Ximbio product webpage
company information
Ximbio
enquiries@ximbio.com
ximbio.com