Anti-PASD1 [2ALCC136] | Ximbio 152546 product information

product summary

company name :

Ximbio

product type :

antibody

product name :

Anti-PASD1 [2ALCC136]

catalog :

152546

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

11153

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

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product information

Name :

Anti-PASD1 [2ALCC136]

Catalogue Number :

152546

Applications :

IHC IF WB

Antigen/Gene or Protein Targets :

PAS domain-containing protein 1 (PASD1), Per Arnt Sim domain 1

Reactivity :

Human

Relevance :

Cancer testis antigens (CTAs) such as PASD1 represent attractive immunotherapy targets, having normal tissue expression that is testis restricted and commonly widespread expression in tumours. PASD1 was originally identified as a target of anti-tumour immune responses in patients with aggressive diffuse large B-cell lymphoma. Subsequent studies have identified PASD1 expression in myeloma patients (both pre- and post-treatment) and in a broad range of solid tumours, 65/160 samples (41%) from 9 histological types. Interestingly a new immune response to PASD1 was detected in a melanoma patient who exhibited a systemic immune response to palliative radiotherapy with regression of nonirradiated lesions, the abscopal effect. Delivery of immunogenic PASD1 peptides using a DNA vaccine was able to induce immune responses in preclinical models, demonstrating its potential for immunotherapy applications. There is relatively little information regarding the function of many CTAs. However, recently PASD1 has been identified as a novel regulator of the circadian clock that interacts with the CLOCK:BMAL1 complex to repress its transcriptional activation. This suggests that PASD1 may contribute to oncogenic transformation through the suppression of circadian rhythms.

Host :

Mouse

Immunogen :

GST-PASD1 recombinant protein containing 195-474 aas of PASD1. Region is common to PASD1a and PASD1b proteins.

Subclass :

IgG

Positive Control :

PASD-1 transfected cells and testis; BT-20 and NCI-H1299 carcinoma lines (negative control: MDA-MB-231, tonsil)

Molecular Weight (kDa) :

100

Myeloma Used :

NS0

Research Area :

Epigenetics & Nuclear Signalling

Notes :

PASD1-1 recognizes the recombinant protein (aa 195–474) present in both PASD1_v1 and PASD1_v2 proteins. Antibody PASD1-1 stains nuclei of a subpopulation of spermatogonia near the basal membrane in testicular tubules, with decreasing intensity of labeling with maturity. IHC stainings may be performed on FFPE and cryostat preparations of cells and tissues. Subclass is IgG2a/IgM mixture despite repeated re-cloning. In WB, expected MW is 87.5 kDa/72.5 kDa, observed MW is ~100 kDa. Recognises both PASD1a and PASD1b proteins.

more info or order :

Ximbio product webpage