antibody

Anti-KAHRP [18.2]

152527

monoclonal

mouse

nonconjugated

18.2

western blot, ELISA, immunocytochemistry

152527

ELISA IF WB

Knob-associated histidine rich protein (KAHRP)

Plasmodium

KAHRP (Plasmodium falciparum knob-associated histidine-rich protein) is a protein expressed in erythrocytes infected by Plasmodium falciparum. KAHRP is a major component of knobs, feature found on Plasmodium falciparum infected erythrocytes, and it contributes to their altered mechanical properties. The sequestration of infected erythrocytes in tissues represents an important factor in the course of fatalities in patients with malaria.

Mouse

Schizont extracts of P.falciparum

IgG2b

P3/NS1/1-Ag4.1

Balb/c

Parasitology

UK Biosafety level: 1 There are 35 SNPs in the KAHRP gene, in IF, mAb 18.2 reacts with isolates 3D7, MAD20, G1, PA17, and K1. Negative IFA results have been obtained with Dd2 and RO33 isolates. Western blotting with schizont extracts from 3D7 parasites shows reactivity of mAb 18.2 with a ~ 90 kDa band. Warning: 3D7 parasites in long term culture may become 18.2 negative by IF – it is advised that proteins prepared for WB are from fresh (<20 generations) parasites. WB conditions: Purify schizont proteinswith MACS column and resolve by SDS-PAGE (4-12% gel, Tris-glycine); blot onto nitrocellulose membrane. Incubate strips from the membrane with mAb 18.2 after blocking (5% non-fat milk in PBS) and detect with HRP-conjugated anti-mouse Ig using 3,3 diaminobenzidine substrate.