Anti-N6-methyladenosine (m6A) [17-3-4-1] | Ximbio 152130 product information

product summary

company name :

Ximbio

product type :

antibody

product name :

Anti-N6-methyladenosine (m6A) [17-3-4-1]

catalog :

152130

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

17-3-4-1

reactivity :

human, mouse

application :

immunoprecipitation

more info or order :

Ximbio product webpage

product information

Name :

Anti-N6-methyladenosine (m6A) [17-3-4-1]

Catalogue Number :

152130

Applications :

IP DB

Antigen/Gene or Protein Targets :

N6-methyladenosine-5'-mono-phosphate

Reactivity :

Human, Mouse and Saccharomyces cerevisiae

Relevance :

N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment.

Host :

Mouse

Immunogen :

Hapten N6-methyladenosine-5'-mono-phosphate conjugated to BSA of all N6-methyladenosine

Subclass :

IgG1 kappa

Myeloma Used :

Sp2/0-Ag14

Recommended Growing Conditions :

DMEM with fetal calf serum (15%), L-Glutamine (2 x 10-3 M), stable Glutamine (2 x 10-3 M), non-essential amino acid solution MEM, 2-mercaptoethanol (5 x 10-5 M)

Strain :

Balb/c

Research Area :

Epigenetics & Nuclear Signalling, Metabolism

Notes :

This antibody recognises N6-methyladenosine in both modified RNA AND DNA (see associated data). Application Note: For immunoprecipitation the best results are obtained by pre-binding the antibody to the beads before washing off unbound immunoglobulin. It is recommended to start with an excess of antibody in order to ensure the Protein G beads are saturated. IP protocol was derived from Dominissini et al., 2013 Nat Protoc. 8(1):176-89.

more info or order :

Ximbio product webpage