Anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9] | Ximbio 152107 product information

product summary

company name :

Ximbio

product type :

antibody

product name :

Anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9]

catalog :

152107

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8348

reactivity :

human

application :

western blot, ELISA, chromatin immunoprecipitation

more info or order :

Ximbio product webpage

citations: 1

Reference
van Welsem T, Korthout T, Ekkebus R, Morais D, Molenaar T, van Harten K, et al. Dot1 promotes H2B ubiquitination by a methyltransferase-independent mechanism. Nucleic Acids Res. 2018;46:11251-11261 pubmed publisher

product information

Name :

Anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9]

Catalogue Number :

152107

Applications :

ChIP ELISA WB

Antigen/Gene or Protein Targets :

Yeast monoubiquityl-histone H2B

Reactivity :

Human, Schizosaccharomyces pombe and Saccharomyces cerevisiae

Relevance :

C-terminus monoubiquitination of histone H2B acts as a signal for downstream processes such as gene regulation, genome packaging, histone modification crosstalk, and DNA repair. How the ubiquitination levels are controlled and how the Ub signal is translated to downstream effects is poorly understood. An antibody that detects K123 monoubiquitinated yeast H2B, homologue to human H2B K120, will enable research to discover the fundamental principles of the regulation and function of monoubiquitination of H2B. The antibody is developed using an ubiquitin-polypeptide antigen based on the amino-acids 115-130 of the yeast H2B protein, linked by a proteolytically stable bond.

Host :

Mouse

Immunogen :

Ub-polypeptide antigen based on amino acids 115 - 130 of yeast H2B

Subclass :

IgG1

Positive Control :

Yeast histone preparation

Molecular Weight (kDa) :

Research Area :

DNA Damage and Repair, Epigenetics & Nuclear Signalling

Notes :

Clone formerly called "H2BK123

more info or order :

Ximbio product webpage