antibody

Anti-Cas9 [7A9-3A3]

152096

monoclonal

mouse

nonconjugated

7A9-3A3

western blot, immunocytochemistry, immunoprecipitation

152096

IF IP WB

Cas9

Bovine, Chicken, Dog, Drosophila, E.coli, Hamster, Horse, Human, Human papilloma virus, Goat, Guinea Pig, Insect, Mink, Mouse, Mammalian, Opossum, Pig, Potoroo, Primate, Rat, Rabbit, Saccharomyces, Virus, Xenopus laevis, Zebrafish, Schizosaccharomyces pombe, Saccharomyces cerevisiae and Saccharomyces frugiperda

Cas9 is an essential endonuclease in Streptococcus pyogenes serotype M1 that is part of that organism’s innate immunity system, or CRISPR (clustered regularly interspaced short palindromic repeat) adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or to help distinguish self versus nonself. Researchers hope to exploit the programmability and precise nature of Cas9 cleavage for more effective recombinant DNA technologies.

Mouse

N-Terminal domain of Cas9.

IgG1 kappa

Genetic Studies Tools

This Antibody recognises both Cas9 and dCas9 (nuclease deficient Cas9).