product summary
company name :
Ximbio
product type :
antibody
product name :
Anti-CLEC2 [AYP1]
catalog :
151913
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2840
reactivity :
human
application :
immunocytochemistry, immunoprecipitation, FACS
more info or order :
Ximbio product webpage
product information
Name :
Anti-CLEC2 [AYP1]
Catalogue Number :
151913
Applications :
FACS IF IP Fn
Antigen/Gene or Protein Targets :
C-type lectin-like receptor 2, CLEC1b
Reactivity :
Human
Relevance :
Patients with rheumatoid arthritis, an inflammatory disease associated with increased microparticle production, have raised plasma levels of microparticles that expressed CLEC-2 but not GPVI. CLEC-2 can be used to monitor platelet-derived microparticles. The observation that microparticles derived from activated platelets retain CLEC-2 but lose GPVI highlights the potential use of measurement of surface expression of platelet receptors to screen for platelet activation in a wide variety of cardiovascular and inflammatory diseases.
Host :
Mouse
Immunogen :
A recombinant extracellular fragment of human CLEC-2 (aa 68-229), which is the extracellular domain of the protein
Subclass :
IgG1
Positive Control :
Platelets
Molecular Weight (kDa) :
26
Myeloma Used :
Sp2/0-Ag14
Recommended Growing Conditions :
Hybridomas were cultured at 37ยบ C and 96% relative humidity in air containing 6% CO2. Cells were grown in complete DMEM + Pen/strep + FCS was used at 10%. Cell density: Cells should be kept between 1-2x105 cells/ml and 5x105 cells/ml.
Research Area :
Cardiovascular, Immunology
Notes :
AYP1 can block the interaction of human CLEC-2 with its endogenous ligand Podoplanin. AYP1 does not cross react with mouse CLEC-2 when tested via FACS. AYP1 does not detect CLEC-2 by western blot. For WB we recommend clone AYP2.
more info or order :
Ximbio product webpage
company information
Ximbio
enquiries@ximbio.com
ximbio.com