Anti-TIGAR [M2-P4H2] | Ximbio 151870 product information

product summary

company name :

Ximbio

product type :

antibody

product name :

Anti-TIGAR [M2-P4H2]

catalog :

151870

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

51283

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

Ximbio product webpage

product information

Name :

Anti-TIGAR [M2-P4H2]

Catalogue Number :

151870

Applications :

ELISA IHC IF WB

Antigen/Gene or Protein Targets :

TP53-induced glycolysis and apoptosis regulator (TIGAR)

Reactivity :

Human

Relevance :

TIGAR (TP53 (tumor protein 53)-induced glycolysis and apoptosis regulator), also known as C12orf5, is a 270 amino acid protein induced by the p53 tumor suppressor pathway that functions to protect against oxidative stress. TIGAR shares sequence similarity with the bisphosphate domain of the fructose- 2,6-bisphosphate degrading enzyme (fructose bisphosphatase or FBPase) of the glycolysis pathway and can thus lower the intracellular levels of fructose- 2,6-bisphosphate. TIGAR specifically functions to block glycolysis, leading the pathway to the pentose phosphate shunt and decreasing the intracellular concentration of reactive oxygen species. This suggests a role for TIGAR in protecting cells from reactive oxygen species that can be DNA damaging and lead to apoptosis.

Host :

Mouse

Immunogen :

Synthetic peptide; IINFEEGREV (amino acids 239-248)

Subclass :

IgG1 kappa

Positive Control :

ELISA- peptide immunogenWestern Blot- Hela Whole cell extract IF- Hela cellsIHC- Human colon carcinoma

Myeloma Used :

P3X63Ag8.653

Recommended Growing Conditions :

Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM + 60 ml Fetal Bovine Serum plus 3ml L-Glutamine(Sigma G7513) + 3ml of Penicillin -Streptomycin (Sigma P0781).

Strain :

Balb/c

Research Area :

Apoptosis and Programmed Cell Death, Cancer, DNA Damage and Repair

more info or order :

Ximbio product webpage