Anti-RSV F Glycoprotein [11-6-F9] | Ximbio 151857 product information

product summary

company name :

Ximbio

product type :

antibody

product name :

Anti-RSV F Glycoprotein [11-6-F9]

catalog :

151857

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

961

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

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product information

Name :

Anti-RSV F Glycoprotein [11-6-F9]

Catalogue Number :

151857

Applications :

ELISA IF Fn WB

Antigen/Gene or Protein Targets :

Human Respiratory Syncytial (RS) virus Fusion glycoprotein

Synonyms :

VP70, F0

Reactivity :

Human and Virus

Relevance :

Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children.The glycoprotein F is located on the surface of viral envelope, its function is to induce fusion of viral envelope with host-cell envelope resulting in syncytium formation. The glycoprotein F (also named VP70, F0 or fusion protein) consists of two components: F1 (also named VPG48) and F2 (also named VGP26) held together by disulphide bonds. The reported molecular weight of the VGP26 component varies between 20 to 26 kDa.

Host :

Mouse

Immunogen :

Gradient-purified RSF-44 virus (subgroup A) UV inactivated for 20 minutes at 20C

Subclass :

IgG1

Positive Control :

Immunoblot: Ag: gradient-purified RS virus (see figure). Indirect immunofluorescence: staining of RSA-2 infected BSC-1 cells

Myeloma Used :

P3X63Ag8.653

Recommended Growing Conditions :

Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).

Strain :

Balb/c

Research Area :

Virology

Notes :

Immunoblot using the reduced and unreduced RS virus shows that 11-6-F9 reacts with the non-reduced form of the virus F protein (VP70). It has a plaque reduction neutralization titer of 10 1.3. Induce in vitro antibody-dependent enhancement of macrophage cells. This antibody has a RS virus 90% plaque neutralization titre of 101.3.and it also has RSV enhancing activity (enhancement index range: 2.5-5.3). Gimenez et al. (1996). Gimenez et al. (1996) was the first report showing that a monoclonal antibody specific for the F protein has enhancing activity.

more info or order :

Ximbio product webpage