Anti-RSV Subgroup B Phosphoprotein [4-14] | Ximbio 151851 product information

product summary

company name :

Ximbio

product type :

antibody

product name :

Anti-RSV Subgroup B Phosphoprotein [4-14]

catalog :

151851

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4-14

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

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product information

Name :

Anti-RSV Subgroup B Phosphoprotein [4-14]

Catalogue Number :

151851

Applications :

ELISA IF WB

Antigen/Gene or Protein Targets :

Human Respiratory Syncytial (RS) virus phosphoprotein VP32

Reactivity :

Virus

Relevance :

Immunoblot assays performed in parallel using as antigen 19 clinical RS virus isolates of subgroups A and B (isolated between 1972 and 1984) showed that this antibody reacted only with the phosphoprotein of RS virus subgroup B (Gimenez et al, 1986). RS virus subgroups A and B circulate concurrently in the human population. Infections with RS virus subgroup A are thought to produce more severe disease than infections with RS virus subgroup B. Consequently, it is beneficial for patient management to know early during an RS virus infection whether the patient is infected with RS virus subgroup A or B. Thus, this antibody is relevant for as a diagnostic tool to determine, soon after infection, the specific RS virus subgroup responsible for the RS virus infection. A diagnostic kit protocol is described in Notes.

Host :

Mouse

Immunogen :

The immunogen was gradient-purified RSN-2 virus (subgroup B) that was then treated with 0.1% SDS at 100°C for 2 min. The procedure used to produce this antibody is described in Gimenez et al. (1984).

Subclass :

IgM

Positive Control :

Immunoblot: Partial purified PVM (lane 2). Partial purified BRS virus (lane 3). Gradient-purified RSN-2 virus (lane1 & 4): 5ug/lane. First antibody: 4-14 antibody (lanes 1,2,3 & 4). Indirect immunofluorescence: staining of RSN-2 infected BSC-1 cells

Myeloma Used :

P3X63Ag8.653

Recommended Growing Conditions :

Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).

Strain :

Balb/c

Research Area :

Virology

Notes :

Diagnostic kit proposal: Antibody : 3-5-18 4-14 Cat number : 151850 151851 Control samples: RS A2 Subgrp A + - RSN-2 Subgrp B + + Patient # 1 sample + - Patient # 2 sample + + Results: Patient # 1 was infected with RSV Subgrp A Patient # 2 was infected with RSV Subgrp B.

more info or order :

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