product summary
company name :
Ximbio
product type :
antibody
product name :
Anti-TIMP1 [2A5]
catalog :
151836
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
350
reactivity :
human
application :
western blot, ELISA, immunohistochemistry
more info or order :
Ximbio product webpage
product information
Name :
Anti-TIMP1 [2A5]
Catalogue Number :
151836
Applications :
ELISA IHC WB
Antigen/Gene or Protein Targets :
TIMP1 (tissue inhibitor of metalloproteinases 1)
Reactivity :
Human
Relevance :
Matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes as well as in disease processes. Tissue inhibitors of metalloproteinases (TIMPs) are the main physiological regulators of the MMPs. The TIMPs are secreted proteins that complex with individual MMPs and regulate the activity of specific MMPs. Together, the MMPs and TIMPs form a complex biological system strictly controlling degradation of ECM. The MMPs and TIMPs have a significant role in facilitating tumour invasion and metastasis. Expression of TIMP1 has been identified in individual studies as prognostic biomarkers in established and locally advanced colorectal cancer.
Host :
Mouse
Immunogen :
Ovalbumin-conjugated synthetic peptide; FQALGDAADIR
Subclass :
IgG1 kappa
Positive Control :
IHC: formalin-fixed, paraffin-embedded colonic adenocarcinoma. Western blot: rhTIMP-1, 400 ng per lane
Myeloma Used :
P3X63Ag8.653
Recommended Growing Conditions :
Dulbecco’s media containing L-Glutamine and 20% Fetal Bovine serum (DH20).
Strain :
Balb/c
Research Area :
Adhesion, Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Protein Degradation
Notes :
IHC: A recommended dilution of 1/8. Recommended pretreatment of antigen retrieval: microwave for 7 minutes at 950W in 0.01M sodium citrate buffer, pH 6.0. Western blot: not reactive with TIMP-2. A recommended dilution of 1/5.
more info or order :
Ximbio product webpage
company information
Ximbio
enquiries@ximbio.com
ximbio.com