antibody

Anti-Ly49B [1A1]

151811

monoclonal

rat

nonconjugated

16633

western blot, immunohistochemistry, immunoprecipitation, FACS

151811

FACS IHC IP WB

Ly49B

Mouse

1A1 specifically recognizes Ly49B in C57 and 129 mice (but not in CBA or BALB mice). Ly49B is the only member of the mouse Ly49 family that has an identifiable ortholog in the rat, suggesting it may have a distinctive and conserved function in murine species. Study using this antibody revealed that Ly49B is not normally expressed on NK cells but instead is expressed on multiple subpopulations of myeloid cells that include granulocytes and macrophages. This antibody can be used for identification of cells expressing Ly49B by flow cytometry or immunohistochemistry and also has utility in immunoprecipitation and Western blotting of Ly49B protein. Candidate anti-Ly49B mAbs were tested for specificity by staining cells transfected with each of the Ly49 molecules known to be expressed in C57 mice. Several mAbs specific for Ly49B were obtained. The mAb 1A1 strongly stained cells transfected with the C57 Ly49B molecule but showed no detectable staining of cells transfected with Ly49A, C, D, E, F, G, H, I, or Q derived from C57 mice. It also failed to stain cells transfected with an HAtagged BALB/c Ly49B molecule that was efficiently stained by anti-HA Ab and with several other Ly49B-specific mAbs.

Rat

RNK cells expressing a C57 Ly49B molecule

IgG1 kappa

F4/80+ve peritoneal cells have uniform high expression

P3/NS1/1-Ag4.1

DMEM 2x nonessential amino acids, 5 x10-5 M 2-ME, and 10% FBS

Immunology