product summary
company name :
Ximbio
product type :
antibody
product name :
Anti-ASAP1 [7B12]
catalog :
151767
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
50807
reactivity :
human, rat
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation
more info or order :
Ximbio product webpage
product information
Name :
Anti-ASAP1 [7B12]
Catalogue Number :
151767
Applications :
ELISA IHC IP WB
Antigen/Gene or Protein Targets :
ASAP1
Reactivity :
Human and Rat
Relevance :
ASAP1 possesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. It may coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. It potentially has involvement in tumour progression, having been shown to promote metastasis formation in vivo and stimulate tumor cell motility, invasiveness, and adhesiveness in vitro. ASAP1 represents a potential target for cancer therapy.
Host :
Mouse
Immunogen :
Recombinant Protein (fragment of human ASAP1 (corresponding nucleotides 977-1532 of KIAA1249) with N-terminal GST tag produced in E.coli strain BL-21)
Subclass :
IgG2b
Positive Control :
Human: HT29 and MDA-MB-231 Rat: ASAP1 is strongly expressed in ASML and weakly expressed in 1AS pancreatic carcinoma cells.
Myeloma Used :
Sp2/0-Ag14
Recommended Growing Conditions :
10% FCS + RPMI + Pen/Strep
Research Area :
Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction
Notes :
Other research areas applicable: Metastasis, cytoskeleton, vesicle trafficking. WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 125 kDa. IHC-P IHC-P: Use a concentration of 5 µg/ml. IHC-Fr IHC-Fr: Use a concentration of 5 µg/ml. IP IP: Use a concentration of 10 µg/ml. ELISA ELISA: Use at an assay dependent concentration.
more info or order :
Ximbio product webpage
company information
Ximbio
enquiries@ximbio.com
ximbio.com