Anti-SIGLEC8 [7C9] | Ximbio 151590 product information

product summary

company name :

Ximbio

product type :

antibody

product name :

Anti-SIGLEC8 [7C9]

catalog :

151590

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

27181

reactivity :

human

application :

ELISA, immunoprecipitation, FACS

more info or order :

Ximbio product webpage

product information

Name :

Anti-SIGLEC8 [7C9]

Catalogue Number :

151590

Applications :

ELISA FACS IP

Antigen/Gene or Protein Targets :

SIGLEC8

Reactivity :

Human

Relevance :

SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-linked sialic acid. and also binds to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.

Host :

Mouse

Immunogen :

Siglec-8-Fc protein, containing entire extracellular region of Human Siglec-8 fused with the Fc region of human IgG1

Subclass :

IgG1

Positive Control :

Siglec-8 CHO cells vs non-transfected CHO cells

Myeloma Used :

Sp2/0-Ag14

Recommended Growing Conditions :

DMEM + 10% Fetal Clone I + 1% penecillin/streptomycin

Strain :

Balb/c

Research Area :

Adhesion, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology

Notes :

Recommended dilution for FACS: Cells (1x10^6) should be incubated with 1ug of antibody/100 ul in 1% BSA in PBS for 30–60 min on ice (Nguyen et al., 2006).

more info or order :

Ximbio product webpage