Anti-SIGLEC5 [1A5] | Ximbio 151589 product information

product summary

company name :

Ximbio

product type :

antibody

product name :

Anti-SIGLEC5 [1A5]

catalog :

151589

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8778

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Ximbio product webpage

product information

Name :

Anti-SIGLEC5 [1A5]

Catalogue Number :

151589

Applications :

ELISA FACS IHC IF IP Fn WB

Antigen/Gene or Protein Targets :

Siglec5

Synonyms :

Sialic Acid Binding Ig Like Lectin 5; Obesity-Binding Protein 2; OB-Binding Protein 2; CD33 Antigen-Like 2; SIGLEC-5; CD33L2; OBBP2; Sialic Acid-Binding Immunoglobulin-Like Lectin 5; CD170 Antigen;

Reactivity :

Human

Relevance :

The Siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Siglec5 (CD170) is a member of the recently-described human CD33-related siglec subgroup of sialic acid binding Ig-like lectins and is expressed on myeloid cells of the hemopoietic system. Similar to other CD33- related siglecs, Siglec-5 contains two tyrosine based motifs in its cytoplasmic tails implicated in signalling functions. The antibody cross reacts with Siglec14.

Host :

Mouse

Immunogen :

Siglec-5-Fc protein, containing entire extracellular region of siglec-5 fused with the Fc region of human IgG1

Subclass :

IgG1

Positive Control :

Siglec5 transfected CHO cells. Apart from Siglec14 (Angata T et al. 2006 FASEB J. 20:1964-73. PMID: 17012248), non-cross-reactive with panel of other Siglec proteins, either expressed on CHO cells or as recombinant proteins.

Myeloma Used :

Sp2/0-Ag14

Recommended Growing Conditions :

DMEM + 10% FetalClone I + 1% penicillin/streptomycin

Research Area :

Adhesion, Cell Type or Organelle Marker, Immunology

Notes :

Recommended dilution for FACS: Cells (1x10^6) should be incubated with 1ug of antibody/100 ul in 1% BSA in PBS for 30–60 min on ice (Nguyen et al., 2006).

more info or order :

Ximbio product webpage