Anti-APC4 [CIV1.1] | Ximbio 151500 product information

product summary

company name :

Ximbio

product type :

antibody

product name :

Anti-APC4 [CIV1.1]

catalog :

151500

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

85441

reactivity :

human

application :

western blot, immunoprecipitation

more info or order :

Ximbio product webpage

product information

Name :

Anti-APC4 [CIV1.1]

Catalogue Number :

151500

Applications :

IP WB

Antigen/Gene or Protein Targets :

Anaphase-promoting complex (APC) 4

Reactivity :

Human

Relevance :

APC4 is a component of the anaphase-promoting complex (APC). The APC complex functions as an ubiquitin ligase which promotes the transition from metaphase to anaphase of the cell cycle. APC4 contains WD repeats and has a molecular weight of approximately 92 kD. APC4 is located in the nucleus during interphase and at the centrosome during metaphase/anaphase.

Host :

Mouse

Immunogen :

C-terminus of APC4

Subclass :

IgG

Positive Control :

Purified APC/C by silver and mass spectrometry

Molecular Weight (kDa) :

Research Area :

Cell Cycle, Protein Degradation

Notes :

CIV1.1 may be used for the affinity purification of the Anaphase Promoting Complex (APC/C) by peptide elution. Suggested Dilution for western blot: 1:500. It is suggested to blot for at least 2 hours (wet transfer) to ensure that APC4 is transferred efficiently. Mass spectrometry was used to confirm that the whole APC/C is co-precipitated. To establish the APC4 specific bands, it is suggested to do an APC4 immunoprecipitation assay showing input, IP and FT and blot for at least 2 hours.

more info or order :

Ximbio product webpage