product summary
company name :
Ximbio
product type :
antibody
product name :
Anti-Melphalan-modified DNA [Amp4/42]
catalog :
151465
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
Amp4/42
application :
ELISA, immunocytochemistry
more info or order :
product information
Name :
Anti-Melphalan-modified DNA [Amp4/42]
Catalogue Number :
151465
Applications :
ELISA IF
Antigen/Gene or Protein Targets :
Melphalan
Reactivity :
Human and Mouse
Relevance :
Antibody Amp4/42 recognises the ring-opened structure that forms when DNA is alkylated by the anti-cancer drug melphalan and is then exposed to alkali. The alkali causes guanine bases alkylated at the N7 position to undergo a so-called ring-opening reaction and Amp4/42 appears to recognise this structure, although recognition may be influenced by the neighbouring DNA sequence. The products of the ring-opening reaction are more resistant to depurination reactions than the original guanine adducts. This antibody was developed to improve detection of melphalan-DNA adducts through its ability to recognise the stabilised adduct. The Amp4/42 clone did not bind to alkali-treated control DNA or to DNA that had been alkylated with melphalan but not exposed to alkali. This antibody can be used for quantification of melphalan adducts in purified DNA by ELISA and for immunofluorescent detection of melphalan-DNA adducts in single cells.
Host :
Rat
Subclass :
IgG2b
Positive Control :
DNA/ melphalan alkylated DNA
Myeloma Used :
Y3.AG.1.2.3
Recommended Growing Conditions :
DMEM + 10%FCS + Glu + Pen/Strep
Research Area :
Cancer
more info or order :
company information
Ximbio
enquiries@ximbio.com
ximbio.com