Anti-TAL1 [BTL73] | Ximbio 151415 product information

product summary

company name :

Ximbio

product type :

antibody

product name :

Anti-TAL1 [BTL73]

catalog :

151415

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6886

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, chromatin immunoprecipitation

more info or order :

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product information

Name :

Anti-TAL1 [BTL73]

Catalogue Number :

151415

Applications :

ChIP ELISA IHC WB

Antigen/Gene or Protein Targets :

TAL1

Reactivity :

Human

Relevance :

T-cell acute lymphocytic leukemia protein 1 (TAL1) is a basic helix-loop-helix transcription factor implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation, including a positive regulation of erythroid differentiation. Diseases associated with TAL1 include leukemia, acute lymphoblastic and precursor t-cell acute lymphoblastic leukemia. A chromosomal aberration involving TAL1 may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL).

Host :

Mouse

Immunogen :

TAL-1 recombinant protein

Subclass :

IgG1

Myeloma Used :

P3/NS1/1-Ag4.1

Recommended Growing Conditions :

It is advised that cells are grown in the following medium:RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT. Ultroser G (**) can be used at 1% if the cells are not growing well.

Research Area :

Cancer, Cell Cycle, Developmental Biology, Epigenetics & Nuclear Signalling, Stem Cell Biology

Application :

Western Blot

Tissue / cell type :

T-acute lymphoblastic leukemia (T-ALL) cell lines

Method :

Nuclear extracts were resolved on 4-12% Bolt Bis-Tris Acrylamide gel (Invitrogen), transferred to nitrocellulose membrane and blocked with 5% low-fat milk powder (Marvel) in PBS. Primary antibody was used 1:1000 in PBS, followed by incubation with the secondary IRDye® 800CW Donkey anti-Mouse IgG (LiCor). Fluorescence was detected using Odyssey imaging system (LI-COR)

Additional notes :

Review provided by Dr Vesna Stanulovic with data from Hoogenkamp lab mambers, University of Birmingham

more info or order :

Ximbio product webpage