product summary
company name :
Ximbio
product type :
antibody
product name :
Anti-Neutrophil elastase [NP57]
catalog :
151398
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1991
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
Ximbio product webpage
product information
Name :
Anti-Neutrophil elastase [NP57]
Catalogue Number :
151398
Applications :
IHC IF IP WB
Antigen/Gene or Protein Targets :
Neutrophil elastase
Reactivity :
Human
Relevance :
Human Neutrophil Elastase (HNE) belongs to the chymotrypsin family of serine proteases able to solubilize fibrous elastin, an important extracellular matrix protein that has the unique property of elastic recoil, and plays a major structural function in lungs, arteries, skin and ligaments. HNE is primarily located in the azurophil granules of polymorphonuclear leukocytes, but has also been detected in nuclear membrane, Golgi complex, ER and the mitochondria of these cells. The enzyme is involved in the tissue destruction and inflammation and implicated in numerous diseases, including emphysema, chronic obstructive pulmonary disease, cystic fibrosis, ischemic-reperfusion injury and rheumatoid arthritis. NP57 is useful for the differential diagnosis of acute leukaemia by APAAP labeling of cell smears. It may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections
Host :
Mouse
Immunogen :
Neutrophil granule proteins
Subclass :
IgG1 kappa
Myeloma Used :
P3/NS1/1-Ag4.1
Recommended Growing Conditions :
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Research Area :
Cancer, Cell Signaling & Signal Transduction, Immunology
Notes :
Ultroser G can be used at 1% if the cells are not growing well.
more info or order :
Ximbio product webpage
company information
Ximbio
enquiries@ximbio.com
ximbio.com