antibody

Anti-CD79a [JCB117]

151375

monoclonal

mouse

nonconjugated

973

western blot, immunohistochemistry

151375

IHC WB

CD79a

CD79a Molecule; Membrane-Bound Immunoglobulin-Associated Protein; Surface IgM-Associated Protein; MB-1 Membrane Glycoprotein; Ig-Alpha; IGA; CD79a Antigen; MB1

Human

The B-cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a (mb1) and CD79b / B29 polypeptides which are non-covalently associated with membrane bound immunoglobulins on B-cells. CD79a first appears at pre B-cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B-cell lymphomas, in B-cell lines, the majority of acute leukemias of precursor B-cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). The induction of tyrosine kinase activity after antigen binding leads to phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signalling. CD79a is widely used as an adjunct to CD20 as a biomarker for normal and neoplastic B-cells in tissues sections.

Mouse

Synthetic peptide of 14 amino acids representing residues 202-216 of the human mb-1 cDNA sequence

IgG1

P3/NS1/1-Ag4.1

RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT

Cancer, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology

Ultroser G can be used at 1% if the cells are not growing well. Western Blot (WB) 1:100-1:500 Immunohistochemistry (Paraffin) (IHC (P)) 1:50-1:100 Flow Cytometry (FACS) 2 ug/test