antibody

Anti-CEACAM5&6 [By114]

151368

monoclonal

mouse

nonconjugated

1048

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

151368

FACS IHC IF IP WB

Carcinoembryonic antigen-related cell adhesion molecules 5 & 6 (CEACAM5, CD66e; CEACAM6, CD66c)CD66 C and E subtypes (NCA)

Carcinoembryonic Antigen Related Cell Adhesion Molecule 5 Meconium Antigen 100; CEA; Carcinoembryonic Antigen; CD66e & c

Human

CEACAM is a member of the carcinoembryonic antigen family (a diagnostic marker for many cancers). Clone By114 reacts with a phosphatidylinositol (PI) linked protein with a molecular mass of 90 kD on granulocyte and squamous epithelium. This antibody is useful in differentiating normal cells from neoplastic cells of granulocyte origin. CD66CE is useful in differentiating normal cells from neoplastic cells of granulocyte origin. By114 may be used to study Paroxysmal Nocturnal Hemoglobinuria (PNH).

Mouse

B cell Lymphoma cells

IgG1 kappa

P3/NS1/1-Ag4.1

RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT

Cancer, Cell Type or Organelle Marker

Ultroser G can be used at 1% if the cells are not growing well. CEACAM5/6 (By114) is recommended for detection of CEACAM5 and CEACAM6 of human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500), immunohistochemistry (including paraffin embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000).