Anti-Cdc20 [AR12.4] | Ximbio 151310 product information

product summary

company name :

Ximbio

product type :

antibody

product name :

Anti-Cdc20 [AR12.4]

catalog :

151310

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1056

reactivity :

human

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Ximbio product webpage

product information

Name :

Anti-Cdc20 [AR12.4]

Catalogue Number :

151310

Applications :

FACS IHC IF IP

Antigen/Gene or Protein Targets :

Cell division cycle protein 20 (cdc20)

Synonyms :

Fizzy protein, CDC20, CDC20A, p55CDC, P55CDC-LSB Human Entrez Gene ID 991 Human SwissProt Q12834 Human Unigene 524947 Human Gene Symbol CDC20 Human Chromosome Location 1p34.2

Reactivity :

Human

Relevance :

Cdc20 (fizzy protein) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.

Host :

Mouse

Immunogen :

Urea-denatured His6 human Cdc20 recombinant protein

Subclass :

IgG1 kappa

Positive Control :

Ramos or HeLa cells. Tonsil or gastric carcinoma.

Molecular Weight (kDa) :

55kDa

Myeloma Used :

P3X63Ag8.653

Recommended Growing Conditions :

DMEM + 5% FCS

Strain :

Balb/c

Research Area :

Cell Cycle, Protein Degradation

Notes :

Cellular Localization: Cytoplasmic Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

more info or order :

Ximbio product webpage