antibody

Anti-CyclinA [E67.1]

151303

monoclonal

mouse

nonconjugated

890

human, mouse, cow

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

151303

FACS IHC IF IP WB

Cyclin A

CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2 Human Entrez Gene ID: 890 Human SwissProt: P20248 Human Unigene: 58974 Human Gene Symbol: CCNA2 Human Chromosome Location: 4q27

Bovine, Human, Mink and Mouse

The anti Cyclin A2, clone E67.1, recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin A is involved in the regulation of the cell cycle and is essential for progression through S phase. Cyclin A protein is absent in cells prior to S-phase, during which its levels increase and peak. Cyclin A is a marker for actively proliferating cells and for cells in S phase. Few other monoclonal antibodies other than E67.1 are able to immunoprecipitate active CDK/Cyclin A H1 kinase from such a wide range of species.

Mouse

Full length bovine Cyclin A protein

IgG2a kappa

K-562 and HeLa Cells

54kDa

DMEM + 5% FCS

Cancer, Cell Cycle, Epigenetics & Nuclear Signalling

Cellular Localization: Predominantly nuclear Suggested dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml);