Anti-RNA polII [CTD 4H8] | Ximbio 151247 product information

product summary

company name :

Ximbio

product type :

antibody

product name :

Anti-RNA polII [CTD 4H8]

catalog :

151247

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5430

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS, chromatin immunoprecipitation, ChIP-Seq

more info or order :

Ximbio product webpage

product information

Name :

Anti-RNA polII [CTD 4H8]

Catalogue Number :

151247

Applications :

ChIP ELISA FACS IHC IF IP WB ChIP-seq

Antigen/Gene or Protein Targets :

RNA Polymerase II

Reactivity :

Human and Saccharomyces cerevisiae

Relevance :

RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. Mutations that directly affect transcription by RNA polymerases rank among the most central mediators of malignant transformation. CTD4H8 can also be used in chromatin immunoprecipitation (ChIP) assays.

Host :

Mouse

Immunogen :

Peptide: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5

Subclass :

IgG1

Positive Control :

HeLa cell line.

Myeloma Used :

Sp2/0-Ag14

Recommended Growing Conditions :

DMEM + 5% FCS

Strain :

Balb/c

Research Area :

Epigenetics & Nuclear Signalling

Notes :

Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as ‘hyper-‘ and ‘hypo-phosphorylated’, respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (though less likely) recognize UN-phosphorylated repeats as there might conceivably be a few of those even in the II0/hyper-phos RPB1 form.

more info or order :

Ximbio product webpage