Anti-Involucrin [SY5] | Ximbio 151163 product information

product summary

company name :

Ximbio

product type :

antibody

product name :

Anti-Involucrin [SY5]

catalog :

151163

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3713

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

more info or order :

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product information

Name :

Anti-Involucrin [SY5]

Catalogue Number :

151163

Applications :

FACS IHC IF IP WB

Antigen/Gene or Protein Targets :

Involucrin

Reactivity :

Dog, Human, Pig and Primate

Relevance :

Clone SY5 recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it.Involucrin is a protein precursor of the epidermal cornified envelope and a differentiation marker of human keratinocytes. SY5 is useful for studies of changes in involucrin expression in pathological studies. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Host :

Mouse

Immunogen :

Purified involucrin from human keratinocytes.

Subclass :

IgG1 kappa

Positive Control :

MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Molecular Weight (kDa) :

66-170

Myeloma Used :

P3X63Ag8

Recommended Growing Conditions :

DMEM + 5% FCS

Strain :

Balb/c

Research Area :

Cancer, Cell Signaling & Signal Transduction

Notes :

There is weak reactivity with Canine, but it does not react with Mouse. Cellular Localization: Cytoplasmic Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistology (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37°C)(Note than enzyme digestion is better than citrate buffer based epitope unmasking)

more info or order :

Ximbio product webpage