Anti-p53 [Pab 1801] | Ximbio 151144 product information

product summary

company name :

Ximbio

product type :

antibody

product name :

Anti-p53 [Pab 1801]

catalog :

151144

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7157

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS, radioimmunoassay, chromatin immunoprecipitation

more info or order :

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product information

Name :

Anti-p53 [Pab 1801]

Catalogue Number :

151144

Applications :

ChIP ELISA FACS IHC IF IP RIA WB

Antigen/Gene or Protein Targets :

p53

Reactivity :

Human

Relevance :

p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. PAb 1801 can be used for the specific detection of human p53 that is synthesised in the presence of p53 from other species.

Host :

Mouse

Immunogen :

Purified p53-b-galactosidase fusion proteins

Subclass :

IgG1

Positive Control :

MDA-MB-231 cell line

Molecular Weight (kDa) :

Myeloma Used :

P3/NS1/1-Ag4.1

Recommended Growing Conditions :

RPMI + 10% FCS

Strain :

Balb/c

Research Area :

Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, DNA Damage and Repair, Epigenetics & Nuclear Signalling

Notes :

PAb 1801 may be used for the specific detection of human p53 which is synthesised in the presence of p53 from other species and is suitable for immunohistochemistry using paraffin embedded samples with pepsin treatment. pAb1801,which recognizes an epitope between amino acids 32 and 79 of both wild-type and mutant p53 (from PMID:12692844) Please note that there are some publications that suggest the PAb 1801 clone also cross reacts proteins unrelated to p53 for example an unknown component of processing bodies (P-bodies) (Wolf et al., 2001. Oncogene. 15;20(11):1307-17. PMID: 11313875; Thomas et al., 2012. PLoS One. 2012; 7(5): e36447 PMID: 22590546). Please ensure that appropriate controls (e.g. MW ladder, treatment to induce p53 expression) are included to ensure the positive signal is from p53.

more info or order :

Ximbio product webpage