Anti-Keratin10 [LH2] | Ximbio 151115 product information

product summary

company name :

Ximbio

product type :

antibody

product name :

Anti-Keratin10 [LH2]

catalog :

151115

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3858

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Ximbio product webpage

product information

Name :

Anti-Keratin10 [LH2]

Catalogue Number :

151115

Applications :

FACS IHC IF IP WB

Antigen/Gene or Protein Targets :

Keratin 10

Synonyms :

BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10 Human Entrez Gene ID 3858 Human SwissProt P13645 Human Unigene 99936 Human Gene Symbol KRT10 Human Chromosome Location 17q21.2

Reactivity :

Human and Mouse

Relevance :

LH2 recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 10 is differentiation specific keratin that is one of the predominant keratins in suprabasal keratinocytes in stratified epthelia. Keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia and is present on all suprabasal keratinocytes. Mutations in keratin 10 cause epidermolytic hyperkeratosis. LH2 does not react with any tumour tissue regardless of histological classification.

Host :

Mouse

Immunogen :

extract of skin from psoriasis patient

Subclass :

IgG1 kappa

Positive Control :

Extract of skin from psoriasis patient

Molecular Weight (kDa) :

56.5kDa

Myeloma Used :

Sp2/0-Ag14

Recommended Growing Conditions :

DMEM + 5% FCS

Strain :

Balb/c

Research Area :

Cell Signaling & Signal Transduction

Notes :

LH2 is monospecific and has high affinity. Cellular Localization: Cytoplasmic Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Immunohistology (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

more info or order :

Ximbio product webpage