protein

Recombinant Human CD274

P8899

50μg/200μg/1 mg

350/700/2100 USD

Recombinant Human CD274

Q9NZQ7

Protein

Human

HEK293 cells

19-239

35-38 kDa

C-terminal His Tag

Greater than 90% as determined by SDS-PAGE.

Western Blot, ELISA

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition.For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.

Measured by its binding ability in a functional ELISA.Immobilized human CD274 at 20 μg/mL. (100 μl/well) can bind human PD1 with a linear range of 0.05-0.1 μg/mL.

B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1

50μg/200μg/1 mg

350/700/2100 USD

1-2 weeks

co-suppression-immune-checkpoint-targets