protein

Recombinant Human TNFA

P4416

50μg/200μg/1 mg

288/576/1728 USD

Recombinant Human TNFA

P01375

Protein

Human

E.Coli

77-233

17.2 kDa

N-terminal His Tag

Greater than 90% as determined by SDS-PAGE.

Western Blot, ELISA

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition.For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin. The ED50 for this effect is 0.1-0.3 ng/mL.

< 1 EU/µg as determined by LAL test.

APC1, APC1 protein, Cachectin, DIF, Differentiation inducing factor, Macrophage cytotoxic factor, Tnf, TNF superfamily member 2, TNF superfamily, member 2, TNF

50μg/200μg/1 mg

288/576/1728 USD

7 days

cytokines