protein

Recombinant Mouse TNFA

P0157

50μg/200μg/1 mg

288/576/1728 USD

Recombinant Mouse TNFA

P06804

Protein

Mouse

E.Coli

80-235

17.2 kDa

N-terminal His Tag

Greater than 90% as determined by SDS-PAGE.

Western Blot, ELISA

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition.For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin. The ED50 for this effect is 20-60 pg/mL.

< 1 EU/µg as determined by LAL test.

APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, tnfa, tnf-a, TNFalpha, TNF-alpha, TNF-alphacachectin, TNFATNF, macrophage-derived

50μg/200μg/1 mg

288/576/1728 USD

7 days

cytokines