ZAP 70 (TCR Zeta Associated Protein kinase 70kD) | US Biological Z0300-03H product information

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product summary

company name :

US Biological

product type :

antibody

product name :

ZAP 70 (TCR Zeta Associated Protein kinase 70kD)

catalog :

Z0300-03H

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

8A43(D1C10E)

reactivity :

human

product information

Catalog Number :

Z0300-03H

Product wo Prefix :

ZAP 70 (TCR Zeta Associated Protein kinase 70kD)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

8A43(D1C10E)

Isotype :

IgG

Desc1 :

Zap-70, a Syk family protein tyrosine kinase expressed in T and NK cells, plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues, presumably by two mechanisms: autophosphorylation and transphosphorylation by the Src family tyrosine kinase, Lck (2-6). Tyrosine phosphorylation of Zap-70 correlates with its increased kinase activity and downstream signaling events. In patients with chronic lymphocytic leukemia (CLL), total Zap-70 expression was shown to be correlated with disease progression and survival (7,8). Applications: Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:200 Western Blot: 1:2000, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mo

Immunogen :

Synthetic peptide corresponding to residues surrounding the N-terminus of human Zap-70.

Specificity :

Detects endogenous levels of total Zap-70. This antibody does not cross-react with other related proteins. Species Crossreactivity: mouse

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 8A43(D1C10E) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding the N-terminus of human Zap-70. Specificity: Detects endogenous levels of total Zap-70. This antibody does not cross-react with other related proteins. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IP WB

Storage Temperature :

-20°C

Reference :

(1) Chu, D.H. et al. (1998) Immunol. Rev. 165, 167–180. (2) Iwashima, M. et al. (1994) Science 263, 1136–1139. (3) Neumeister, E.N. et al. (1995) Mol. Cell Biol. 15, 3171–3178. (4) Chan, A.C. et al. (1995) EMBO J. 14, 2499–2508. (5) Williams, B.L. et al. (1999) EMBO J. 18, 1832–1844. (6) Di Bartolo, V. et al. (1999) J. Biol. Chem. 274, 6285–6294. (7) Wiestner, A. et al. (2003) Blood 101, 4944–4951. (8) Crespo, M. et al. (2003) N. Engl. J. Med. 348, 1764–1775.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/33/Z0300-03H_1.jpg